引用本文:
洪涛, 王秋云, 范军, 陈文静, 郑盛润, 章伟光. 流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别[J]. 应用化学,
2013, 30(9): 1096-1098.
doi:
10.3724/SP.J.1095.2013.30012
Citation: HONG Tao, WANG Qiuyun, FAN Jun, CHEN Wenjing, ZHENG Shengrun, ZHANG Weiguang. Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance[J]. Chinese Journal of Applied Chemistry, 2013, 30(9): 1096-1098. doi: 10.3724/SP.J.1095.2013.30012
Citation: HONG Tao, WANG Qiuyun, FAN Jun, CHEN Wenjing, ZHENG Shengrun, ZHANG Weiguang. Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance[J]. Chinese Journal of Applied Chemistry, 2013, 30(9): 1096-1098. doi: 10.3724/SP.J.1095.2013.30012
流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别
摘要:
采用流动注射-石英晶体微天平(FIA-QCM)和紫外光谱法研究了牛/羊血清蛋白(BSA和GSA)与扁桃酸的手性识别。在QCM表面,BSA和GSA的浓度分别为4.9×10-12和8.8×10-12mol/cm2。血清蛋白与R-扁桃酸(R-MA)作用引起QCM振动频率变化值比S-扁桃酸(S-MA)大;相应紫外结合常数也比与S-MA大,说明血清蛋白对R-MA的作用强于与S-对映体。从识别因子分析,GSA对扁桃酸的手性识别能力优于BSA。
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关键词:
- 血清蛋白
- / 扁桃酸
- / 流动注射-石英晶体微天平
- / 紫外光谱
- / 识别因子
English
Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance
Abstract:
Interactions of mandelic acid(MA) and bovine/goat serum albumins(BSA/GSA) have been investigated by FIA-QCM and UV-Vis spectroscopy, respectively. Based on frequency shift of quartz crystal,surface concentrations of immobilized BSA and GSA were 4.9 × 10-12 and 8.8 × 10-12 mol/cm2,respectively. After injecting mandelic acid into FIA-QCM system,frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer,indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA. The UV binding constants for SA with S-MA are smaller than those with R-enantiomer. Taking into account the discrimination factors (αQCM and αUV),GSA shows a better chiral recognition with mandelic acid than BSA.
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