引用本文:
仲蕙, 方正, 邹宝华, 李昕, 郭凯. 无溶剂体系中固定化脂肪酶Candida sp.99-125催化合成油酸低碳醇酯[J]. 应用化学,
2013, 30(9): 1030-1035.
doi:
10.3724/SP.J.1095.2013.20496
Citation: ZHONG Hui, FANG Zheng, ZOU Baohua, LI Xin, GUO Kai. Immobilized-Lipase(Candida sp. 99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems[J]. Chinese Journal of Applied Chemistry, 2013, 30(9): 1030-1035. doi: 10.3724/SP.J.1095.2013.20496
Citation: ZHONG Hui, FANG Zheng, ZOU Baohua, LI Xin, GUO Kai. Immobilized-Lipase(Candida sp. 99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems[J]. Chinese Journal of Applied Chemistry, 2013, 30(9): 1030-1035. doi: 10.3724/SP.J.1095.2013.20496
无溶剂体系中固定化脂肪酶Candida sp.99-125催化合成油酸低碳醇酯
English
Immobilized-Lipase(Candida sp. 99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems
Abstract:
The esterification between oleic acid and alkyl alcohols in solvent-free systems were catalyzed by an immobilized lipase from Candida sp.99-125. The effects of several factors including enzyme concentration,temperature,molar ratio of oleic acid to alkyl alcohols,and structure of alcohols have been also investigated. The results indicated that the reactions catalyzed by lipase at 20℃ with the presence of 3% (mass fraction) lipase,a 1:1 molar ratio of oleic acid to alcohols,afforded products in high yields. Methanol has certain toxicity on the activity of the lipase. The enzyme showed high selectivity to primary alcohols and low selectivity to secondary and tertiary alcohols because of the sterical effect. It showed high activity to long chain fatty acids and low activity to polybasic acids and polyhydric alcohols with branched chain. The lipase showed no appreciable loss in activity after being continuously operated for 5 times. The enzymatic synthesis gave purer products,compared with the conventional chemical system.
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Key words:
- enzymatic catalysis
- / immobilized lipase
- / esterification
- / oleates
- / solvent-free system
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