基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用

引用本文: 施露露, 洪欢欢, 陈安琪, 唐燕, 张涛, 闻路红, 赵鹏. 基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用[J]. 分析化学, 2023, 51(3): 348-355. doi: 10.19756/j.issn.0253-3820.221368 shu

Citation: SHI Lu-Lu, HONG Huan-Huan, CHEN An-Qi, TANG Yan, ZHANG Tao, WEN Lu-Hong, ZHAO Peng. Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 348-355. doi: 10.19756/j.issn.0253-3820.221368 shu

基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用

施露露 ^1,2, ,
洪欢欢 ^3, ,
陈安琪 ^3, ,
唐燕 ^3, ,
张涛 ^1, ,
闻路红 ^{2,3,
,} ,
赵鹏 ^{3,
,}

1.
中国科学院上海技术物理研究所, 上海 200083;
2.
宁波华仪宁创智能科技有限公司, 宁波 315100;
3.
宁波大学高等技术研究院, 宁波 315211

通讯作者: 闻路红,E-mail:wenluhong@nbu.edu.cn; 赵鹏,E-mail:zhaopeng@nbu.edu.cn

基金项目:
国家重点研发计划项目(No.2018YFC16000600)、浙江省重点研发计划项目(No.2020C2023)、宁波市“揭榜挂帅”重大攻关项目(No.20211ZDYF020179)和浙江省博士后科研项目择优资助项目(No.ZJ2021003)资助。

摘要: 脉冲直流电喷雾离子化质谱(PDESI-MS)分析具有原位、实时、快速和可分析微量样品等优点。然而,目前的PDESI-MS系统需手动操作进行样品处理和质谱进样,存在操作繁琐、步骤多、误差大和准确度低等问题。本工作研制了一套高通量、全自动化和一体化的PDESI-MS系统,探索了其在真核生物(单个膀胱癌细胞)和原核生物(细菌单菌落)中小分子代谢物原位检测分析中的应用。结果表明,本自动化PDESI-MS系统可有效检测不同菌种代谢物,实现了不同类型大肠杆菌的分类定型;同时,皮升级液滴产生技术使其可应用于单细胞检测。自动化PDESI-MS系统可实现高精度、高密度、高重复度和高自动化的原位样品萃取,特别是微量小体积样品的分析,在食品安全、再生医学、药物筛选和临床治疗等领域具有极大的应用潜力和良好的发展前景。

关键词:

English

Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application

1.
Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China;
2.
China Innovation Instrument Co. Ltd., Ningbo 315100, China;
3.
The Research Institute of Advanced Technologies, Ningbo University, Ningbo 315211, China

Corresponding author: WEN Lu-Hong, ; ZHAO Peng,

Received Date: 21 July 2022
Revised Date: 26 November 2022
Fund Project:
Supported by the National Key Research and Development Program (No. 2018YFC16000600), the Zhejiang Province Welfare Technology Applied Research Project (No. 2020C02023), the Ningbo City Major Scientific and Technological Program of China (No. 20211ZDYF020179) and the Preferred Postdoctoral Research Projects Foundation of Zhejiang Province (No. ZJ2021003).

Abstract: Pulsed direct current electrospray ionization mass spectrometry (PDESI-MS) offers many advantages such as in situ, real-time, fast, and microvolume sample analysis. However, the current PDESI-MS system requires manual operation for sample processing and mass spectrometry injection, which have many disadvantages such as cumbersome operation, many steps, large errors and low accuracy. Herein, a high-throughput, fully automated and integrated PDESI-MS system was developed, and an in situ detection for small molecule metabolites in eukaryotes (single bladder cancer cells) and prokaryotes (single colonies of bacteria) was explored. The results showed that the automated PDESI-MS system could effectively detect metabolites of different strains and realize the classification and stereotyping of different types of Escherichia coli. In addition, the picoliter droplet generation technology allowed it to be applied to single cell detection. The automated PDESI-MS system could achieve high precision, high density, high repeatability and high automation for in situ sample extraction, especially in detection and analysis of small samples, and showed great application potential and development prospects in food safety, regenerative medicine, drug screening, clinical treatment and other fields.

Key words:

Pulsed direct current electrospray ionization
/ Automation
/ In situ detection
/ Small droplets

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用

通讯作者: 闻路红,E-mail:wenluhong@nbu.edu.cn; 赵鹏,E-mail:zhaopeng@nbu.edu.cn

English

Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application

Corresponding author: WEN Lu-Hong, ; ZHAO Peng,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用

通讯作者: 闻路红,E-mail:wenluhong@nbu.edu.cn; 赵鹏,E-mail:zhaopeng@nbu.edu.cn

English

Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application

Corresponding author: WEN Lu-Hong, ; ZHAO Peng,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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