表面增强拉曼散射纳米针尖用于单细胞内环境的检测研究

引用本文: 陈颖, 梅荣超, 王运庆, 刘万卉, 陈令新. 表面增强拉曼散射纳米针尖用于单细胞内环境的检测研究[J]. 分析化学, 2023, 51(3): 356-363. doi: 10.19756/j.issn.0253-3820.221167 shu

Citation: CHEN Ying, MEI Rong-Chao, WANG Yun-Qing, LIU Wan-Hui, CHEN Ling-Xin. Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 356-363. doi: 10.19756/j.issn.0253-3820.221167 shu

表面增强拉曼散射纳米针尖用于单细胞内环境的检测研究

陈颖 ^1,2, ,
梅荣超 ^2, ,
王运庆 ^{2,3,
,} ,
刘万卉 ^1, ,
陈令新 ^{2,3,
,}

1.
烟台大学药学院, 分子药理和药物评价教育部重点实验室(烟台大学), 新型制剂与生物技术药物研究山东省高校协同创新中心, 烟台 264005;
2.
中国科学院烟台海岸带研究所, 中国科学院海岸带环境过程与生态修复重点实验室, 烟台 264003;
3.
山东省海岸带环境过程重点实验室, 烟台 264003

通讯作者: 王运庆,E-mail:yqwang@yic.ac.cn; 陈令新,E-mail:lxchen@yic.ac.cn

基金项目:
国家自然科学基金项目(Nos.42076199,21976209)资助。

摘要: 表面增强拉曼散射(SERS)纳米针尖是一类单细胞分析新技术,在细胞内环境检测和细胞生理功能研究等方面具有良好的应用潜力。由于SERS纳米针尖可负载的贵金属粒子数量少,因此,筛选和修饰高SERS增强能力的纳米粒子是确保其检测灵敏度的关键。本研究制备了一种核-卫星结构的Au纳米粒子,单颗粒信号较传统Au纳米球和Au纳米星显著提高。将此粒子涂覆在尖端直径约为200 nm的玻璃毛细管表面,形成SERS纳米针尖,进一步功能化修饰靶标敏感型拉曼报告分子,使其具备检测微区环境中pH值和O₂的能力。作为应用性能考察,采用SERS纳米针尖实现了单个HL-7702细胞内pH值和缺氧状态监测。本研究解决了传统颗粒态SERS探针用于细胞分析面临的随机聚集和难以精确定位等瓶颈问题,为单细胞内环境检测分析提供了一种新的分析工具。

关键词:

English

Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip

1.
Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, School of Pharmacy, Yantai University, Yantai 264005, China;
2.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
3.
Shandong Key Laboratory of Coastal Environmental Processes, Yantai 264003, China

Corresponding author: WANG Yun-Qing, ; CHEN Ling-Xin,

Received Date: 06 April 2022
Revised Date: 13 October 2022
Fund Project:
Supported by the National Natural Foundation of China (Nos. 42076199, 21976209).

Abstract: Surface-enhanced Raman scattering (SERS) nanotip is a kind of new technique for single cell analysis, which shows excellent application potential in detection of intracellular environment and study of cell physiological function. Due to the small number of noble metal particles that can be loaded on SERS nanotips, screening and modifying nanoparticles with high SERS enhancement ability is the key to ensure their detection sensitivity. In this study, core-satellite Au nanoparticles were prepared, and the single particle signal was significantly higher than that of traditional Au nanospheres and Au nanostars. The particle was coated on the surface of glass capillary with tip diameter of about 200 nm to form SERS nanotips, which further functionalized and modified with target-sensitive Raman reporter molecules, enabling them to detect pH value and O₂ content in micro-area environment. As application performance investigation, SERS nanotips realized pH value and anoxic state monitoring in single HL-7702 cell. This study solved the bottleneck issues such as random aggregation and difficulty in precise localization faced by traditional granular SERS probes for cell analysis, providing a new analytical tool for detection and analysis of single cell environment.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

表面增强拉曼散射纳米针尖用于单细胞内环境的检测研究

通讯作者: 王运庆,E-mail:yqwang@yic.ac.cn; 陈令新,E-mail:lxchen@yic.ac.cn

English

Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip

Corresponding author: WANG Yun-Qing, ; CHEN Ling-Xin,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

表面增强拉曼散射纳米针尖用于单细胞内环境的检测研究

通讯作者: 王运庆,E-mail:yqwang@yic.ac.cn; 陈令新,E-mail:lxchen@yic.ac.cn

English

Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip

Corresponding author: WANG Yun-Qing, ; CHEN Ling-Xin,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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