基于硫酸软骨素和聚乙二醇协同抗污的电化学传感界面灵敏检测弧菌<i>tlh</i>基因片段

引用本文: 张宇宇, 黄雅玥, 曾晖, 杨涛, 罗细亮. 基于硫酸软骨素和聚乙二醇协同抗污的电化学传感界面灵敏检测弧菌tlh基因片段[J]. 分析化学, 2022, 50(7): 1041-1047. doi: 10.19756/j.issn.0253-3820.221021 shu

Citation: ZHANG Yu-Yu, HUANG Ya-Yue, ZENG Hui, YANG Tao, LUO Xi-Liang. Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus[J]. Chinese Journal of Analytical Chemistry, 2022, 50(7): 1041-1047. doi: 10.19756/j.issn.0253-3820.221021 shu

基于硫酸软骨素和聚乙二醇协同抗污的电化学传感界面灵敏检测弧菌tlh基因片段

张宇宇 ^1, ,
黄雅玥 ^1, ,
曾晖 ^1, ,
杨涛 ^{1,
,} ,
罗细亮 ^{2,
,}

1.
中山大学化学工程与技术学院, 珠海 519082;
2.
青岛科技大学化学与分子工程学院, 青岛 266061

通讯作者: 杨涛,E-mail:yangtao25@mail.sysu.edu.cn; 罗细亮,E-mail:xiliangluo@qust.edu.cn

基金项目:
国家自然科学基金项目(No.21675092)、广州市产学研一体化专项研究项目(No.201604016008)和佛山市南海经济技术促进局项目(No.20177611071010008)资助。

摘要: 通过简单的电化学方法合成高导电性和含有丰富羧基的聚氨基苯甲酸(Poly (m-aminobenzoic acid),PABA),随后通过共价键合的方式在修饰界面引入具有良好亲水性和生物相容性的聚乙二醇(Polyethylene glycol,PEG)和硫酸软骨素(Chondroitin sulfate,CS),构建了一种用于检测副溶血性弧菌tlh基因片段的抗污染电化学生物传感平台。导电聚合物与抗污染材料的结合,在赋予了传感界面抵抗非特异性物质吸附性能的同时,既保持了传感器对弧菌特异性DNA片段的高灵敏度、高选择性的检测能力,又增强了传感界面的稳定性。在最佳条件下,采用此生物传感器对tlh基因片段进行检测,线性范围为1.0×10^-20~1.0×10^-8 mol/L,检出限(S/N=3)低至3.3×10^-21 mol/L。与PABA、单一PEG以及单一CS修饰电极相比,在CS和PEG的协同作用下,此生物传感平台具有更优异的抗蛋白吸附性能。

关键词:

English

Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus

ZHANG Yu-Yu ^1, ,
HUANG Ya-Yue ^1, ,
ZENG Hui ^1, ,
YANG Tao ^{1,
,} ,
LUO Xi-Liang ^{2,
,}

1.
School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China;
2.
School of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266061, China

Corresponding author: YANG Tao, ; LUO Xi-Liang,

Received Date: 12 January 2022
Revised Date: 29 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.21675092), the Special Project on the Integration of Industry, Education and Research of Guangzhou (No.201604016008) and the Foshan Nanhai Economic and Technological Promotion Bureau Project (No.20177611071010008).

Abstract: Foodborne pathogens are the main cause of seafood poisoning in the world, and the traditional electrochemical detection methods have always suffered from nonspecific adsorption and serious biological fouling in the practical complex samples. Therefore, it is urgent to develop antifouling and sensitive biosensor platforms for detecting foodborne pathogens in complex matrix. In this study, by integrating polyethylene glycol (PEG) with good hydrophilicity and chondroitin sulfate (CS) with excellent biocompatibility, an electrochemical DNA sensing interface for detection of the tlh gene segment of Vibrio parahaemolyticus based on synergistic antifouling of PEG and CS was constructed. Compared with sole poly(m-aminobenzoic acid) modified glassy carbon electrode (PABA/GCE), PEG/PABA/GCE and CS/PABA/GCE, CS/PEG/PABA/GCE exhibited many advantages such as excellent anti-protein (single protein and complex skim milk) adsorption performance and stability. Moreover, the sensing interface showed high sensitivity and satisfactory selectivity to the tlh gene segment in the range of 1.0×10^-20-1.0×10^-8 mol/L with a detection limit of 3.3×10^-21 mol/L.

Key words:

1. [1]
  XUE L, ZHENG L, ZHANG H L, JIN X, LIN J H. Sens. Actuators, B, 2018, 265:318-325.XUE L, ZHENG L, ZHANG H L, JIN X, LIN J H. Sens. Actuators, B, 2018, 265:318-325.
2. [2]
  ZHU P, GAO W F, HUANG H L, JIANG J P, CHEN X F, FAN J Z, YAN X J. Food Anal. Method, 2018, 11:2076-2084.ZHU P, GAO W F, HUANG H L, JIANG J P, CHEN X F, FAN J Z, YAN X J. Food Anal. Method, 2018, 11:2076-2084.
3. [3]
  NIGRO O D, STEWARD G F. J. Microbiol. Methods, 2015, 111:24-30.NIGRO O D, STEWARD G F. J. Microbiol. Methods, 2015, 111:24-30.
4. [4]
  BAYAT M, KHABIRI A, HEMATI B. Can. J. Infect. Dis. Med. Microbiol., 2019, 2019:4164982.BAYAT M, KHABIRI A, HEMATI B. Can. J. Infect. Dis. Med. Microbiol., 2019, 2019:4164982.
5. [5]
  TABATABAEI M S, ISLAM R, AHMED M. Anal. Chim. Acta, 2021, 1143:250-266.TABATABAEI M S, ISLAM R, AHMED M. Anal. Chim. Acta, 2021, 1143:250-266.
6. [6]
  LI Y, LIU H M, HUANG H, DENG J, FANG L C, LUO J, ZHANG S, HUANG J, LIANG W B, ZHENG J S. Biosens. Bioelectron., 2020, 147:111752.LI Y, LIU H M, HUANG H, DENG J, FANG L C, LUO J, ZHANG S, HUANG J, LIANG W B, ZHENG J S. Biosens. Bioelectron., 2020, 147:111752.
7. [7]
  LIU Ting, LIN Xiao-Hui, CHEN Qian, BIAN Xiao-Jun, YAN Juan. Chin. J. Anal. Chem., 2022, 50(1):127-135.刘婷,林晓惠,陈谦,卞晓军,颜娟.分析化学, 2022, 50(1):127-135.
8. [8]
  QIU Q M, CHEN H Y, YING S N, SHARIF S, YOU Z H, WANG Y X, YING Y B. Microchim. Acta, 2019, 186(2):93.QIU Q M, CHEN H Y, YING S N, SHARIF S, YOU Z H, WANG Y X, YING Y B. Microchim. Acta, 2019, 186(2):93.
9. [9]
  WANG M, ZENG J, WANG J Q, WANG X, WANG Y, GAN N. Microchim. Acta, 2021, 188(8):244.WANG M, ZENG J, WANG J Q, WANG X, WANG Y, GAN N. Microchim. Acta, 2021, 188(8):244.
10. [10]
  ZHANG Qian-Wen, CHEN Qian, BIAN Xiao-Jun, YAN Juan. Chin. J. Anal. Chem., 2021, 49(8):1289-1299.张倩雯,陈谦,卞晓军,颜娟.分析化学, 2021, 49(8):1289-1299.
11. [11]
  ZHANG Z G, ZHOU J, DU X. Micromachines, 2019, 10(4):222.ZHANG Z G, ZHOU J, DU X. Micromachines, 2019, 10(4):222.
12. [12]
  LIN P H, LI B R. Analyst, 2020, 145(4):1110-1120.LIN P H, LI B R. Analyst, 2020, 145(4):1110-1120.
13. [13]
  CUI M, SONG Z, WU Y, GUO B, FAN X, LUO X L. Biosens. Bioelectron., 2016, 79:736-741.CUI M, SONG Z, WU Y, GUO B, FAN X, LUO X L. Biosens. Bioelectron., 2016, 79:736-741.
14. [14]
  HUI N, SUN X, SONG Z, NIU S, LUO X L. Biosens. Bioelectron., 2016, 86:143-149.HUI N, SUN X, SONG Z, NIU S, LUO X L. Biosens. Bioelectron., 2016, 86:143-149.
15. [15]
  CUI M, WANG Y, JIAO M, JAYACHANDRAN S, WU Y, FAN X, LUO X L. ACS Sens., 2017, 2(4):490-494.CUI M, WANG Y, JIAO M, JAYACHANDRAN S, WU Y, FAN X, LUO X L. ACS Sens., 2017, 2(4):490-494.
16. [16]
  WANG G, HAN R, SU X, LI Y, XU G, LUO X L. Biosens. Bioelectron., 2017, 92:396-401.WANG G, HAN R, SU X, LI Y, XU G, LUO X L. Biosens. Bioelectron., 2017, 92:396-401.
17. [17]
  XU Z Y, HAN R, LIU N Z, GAO F X, LUO X L. Sens. Actuators, B, 2020, 319:128253.XU Z Y, HAN R, LIU N Z, GAO F X, LUO X L. Sens. Actuators, B, 2020, 319:128253.
18. [18]
  XIA Y Q, ADIBNIA V, SHAN C C, HUANG R L, QI W, HE Z M, XIE G J, OLSZEWSKI M, DE CRESCENZO G, MATYJASZEWSKI K, BANQUY X, SU R X. Langmuir, 2019, 35(48):15535-15542.XIA Y Q, ADIBNIA V, SHAN C C, HUANG R L, QI W, HE Z M, XIE G J, OLSZEWSKI M, DE CRESCENZO G, MATYJASZEWSKI K, BANQUY X, SU R X. Langmuir, 2019, 35(48):15535-15542.
19. [19]
  YE H J, HAN M Y, HUANG R L, SCHMIDT T A, QI W, HE Z M, MARTIN L L, JAY G D, SU R X, GREENE G W. ACS Appl. Mater. Interfaces, 2019, 11(20):18090-18102.YE H J, HAN M Y, HUANG R L, SCHMIDT T A, QI W, HE Z M, MARTIN L L, JAY G D, SU R X, GREENE G W. ACS Appl. Mater. Interfaces, 2019, 11(20):18090-18102.
20. [20]
  CHEN L H, LV S L, LIU M C, CHEN C F, SHENG J L, LUO X L. ACS Appl. Nano Mater., 2018, 1(6):2489-2495.CHEN L H, LV S L, LIU M C, CHEN C F, SHENG J L, LUO X L. ACS Appl. Nano Mater., 2018, 1(6):2489-2495.
21. [21]
  ZHAO S Y, ZHOU Y X, WEI L, CHEN L H. Anal. Chim. Acta, 2020, 1126:91-99.ZHAO S Y, ZHOU Y X, WEI L, CHEN L H. Anal. Chim. Acta, 2020, 1126:91-99.
22. [22]
  YANG T, CHEN H Y, QIU Z W, YU R Z, LUO S Z, LI W H, JIAO K. ACS Appl. Mater. Interfaces, 2018, 10(5):4540-4547.YANG T, CHEN H Y, QIU Z W, YU R Z, LUO S Z, LI W H, JIAO K. ACS Appl. Mater. Interfaces, 2018, 10(5):4540-4547.
23. [23]
  ZHU W C, HUANG H, GAO X C, MA H Y. Mater. Sci. Eng. C, 2014, 45:21-28.ZHU W C, HUANG H, GAO X C, MA H Y. Mater. Sci. Eng. C, 2014, 45:21-28.
24. [24]
  WANG S, MA Y H, WANG Y, JIAO M X, LUO X L, CUI M. Colloids Surf., B, 2020, 186:110706.WANG S, MA Y H, WANG Y, JIAO M X, LUO X L, CUI M. Colloids Surf., B, 2020, 186:110706.
25. [25]
  LIU N Z, SONG J Y, LU Y W, DAVIS J J, GAO F X, LUO X L. Anal. Chem., 2019, 91(13):8334-8340.LIU N Z, SONG J Y, LU Y W, DAVIS J J, GAO F X, LUO X L. Anal. Chem., 2019, 91(13):8334-8340.
26. [26]
  HUI N, SUN X T, NIU S Y, LUO X L. ACS Appl. Mater. Interfaces, 2017, 9(3):2914-2923.HUI N, SUN X T, NIU S Y, LUO X L. ACS Appl. Mater. Interfaces, 2017, 9(3):2914-2923.
27. [27]
  FIGUERO-MIRANDA G, WU C T, ZHANG Y T, NOERBEL L, LO Y, TANNER J A, ELLING L, OFFENHAUSSER A, MAYER D. Bioelectrochemistry, 2020, 136:107589.FIGUERO-MIRANDA G, WU C T, ZHANG Y T, NOERBEL L, LO Y, TANNER J A, ELLING L, OFFENHAUSSER A, MAYER D. Bioelectrochemistry, 2020, 136:107589.

扫一扫看文章

计量

PDF下载量: 6
文章访问数: 583
HTML全文浏览量: 112

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于硫酸软骨素和聚乙二醇协同抗污的电化学传感界面灵敏检测弧菌tlh基因片段

通讯作者: 杨涛,E-mail:yangtao25@mail.sysu.edu.cn; 罗细亮,E-mail:xiliangluo@qust.edu.cn

English

Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus

Corresponding author: YANG Tao, ; LUO Xi-Liang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于硫酸软骨素和聚乙二醇协同抗污的电化学传感界面灵敏检测弧菌tlh基因片段

通讯作者: 杨涛,E-mail:yangtao25@mail.sysu.edu.cn; 罗细亮,E-mail:xiliangluo@qust.edu.cn

English

Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus

Corresponding author: YANG Tao, ; LUO Xi-Liang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs