细胞顶空气中典型醛酮化合物定量分析方法研究

引用本文: 尹志红, 陈钊铭, 陈星, 杨子峰, 李征途, 叶枫, 张志娟, 方明亮, 李雪. 细胞顶空气中典型醛酮化合物定量分析方法研究[J]. 分析化学, 2021, 49(8): 1375-1383. doi: 10.19756/j.issn.0253-3820.211072 shu

Citation: YIN Zhi-Hong, CHEN Zhao-Ming, CHEN Xing, YANG Zi-Feng, LI Zheng-Tu, YE Feng, ZHANG Zhi-Juan, FANG Ming-Liang, LI Xue. Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell[J]. Chinese Journal of Analytical Chemistry, 2021, 49(8): 1375-1383. doi: 10.19756/j.issn.0253-3820.211072 shu

细胞顶空气中典型醛酮化合物定量分析方法研究

1.
暨南大学质谱仪器与大气环境研究所, 广州 510632;
2.
广东省大气污染在线源解析系统工程技术研究中心, 广州 510632;
3.
广州医科大学呼吸疾病国家重点实验室, 广州 511436;
4.
广州医科大学附属第一医院, 国家呼吸系统疾病临床医学研究中心, 广州 510120;
5.
河南中医药大学药学院, 郑州 450008;
6.
南洋理工大学土木与环境工程学院, 新加坡 637457

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

基金项目:
广东特支计划本土创新创业团队项目（No.2019BT02Z546）、呼吸疾病国家重点实验室开放课题项目（No.SKLRD-OP-201913）和中国科学院地球科学领域核心关键技术攻关预研项目（No.282021000003）资助。

摘要: 采用2，4-二硝基苯肼衍生柱收集挥发性醛酮类组分，转化为稳定的醛酮2，4-二硝基苯腙有机物，采用超高效液相色谱-高分辨质谱联用技术进行分析，建立了22种典型醛酮类化合物定量分析方法。2，4-二硝基苯腙有机物在高（40~1000 μg/L）、中（8~400 μg/L）、低（0.08~8 μg/L）浓度范围内标准曲线线性良好，相关系数R²>0.998，检出限范围为0.003~0.09 μg/L；高、中、低浓度范围的标准溶液的日内及日间精密度（RSD）均小于10%；回收率在95.1%~109.6%之间，RSD≤5.9%。将本方法用于分析A549细胞以及甲型H1N1流感病毒（IAV）感染的A549细胞顶空气中醛酮类物质，检测到19种醛酮类物质，通过正交偏最小二乘-判别分析法分析检测结果，发现两组醛酮代谢物存在显著差别，并筛选出11种潜在的醛酮生物标记物

关键词:

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

1.
Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
2.
Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Guangzhou 510632, China;
3.
State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 511436, China;
4.
National Clinical Research Center for Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China;
5.
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, China;
6.
School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 637457, Singapore

Corresponding author: LI Xue, tamylee@jnu.edu.cn

Received Date: 26 January 2021
Revised Date: 01 April 2021
Fund Project:
Supported by the "Guangdong Special Support Plan" Local Innovation and Entrepreneurship Team Project (No.2019BT02Z546), the Open Project of the State Key Laboratory of Respiratory Diseases (No.SKLRD-OP-201913), the Pre-research Project of Core Key Technologies in the Field of Earth Sciences, Chinese Academy of Sciences (No.282021000003).

Abstract: Aldehydes and ketones in breath can reflect the oxidative stress response of the cells in the body. A better understanding of the aldehydes and ketones emitted from the cells is the key to revealing the mechanism of breath test for the diagnosis of oxidative stress-related diseases. In this study, a quantitative method was developed for 22 typical aldehyde and ketone metabolites. Standard compounds were transformed into stable DNPH-carbonyl adducts by using 2,4-dinitrophenylhydrazine (DNPH) impregnated cartridge. These adducts were further eluted and analyzed by ultra high performance liquid chromatography coupled with high resolution mass spectrometry. The standard curves of 22 DNPH-carbonyl adducts showed linearity at high (40-1000 μg/L), medium (8-400 μg/L) and low (0.08-8 μg/L) levels, respectively. The coefficient of correlation (R²) was >0.998 and the detection limits were 0.003-0.09 μg/L. The method demonstrated good precision, e.g. intra-day RSD <3.92% and inter-day RSD <5.3%, as well as satisfactory recoveries of 95.1%-109.6% (RSD<5.9%). The method was further applied to the analysis of aldehydes and ketones in the headspace of A549 cells and influenza A (H1N1) infected A549 cells. The experimental data were analyzed by using orthogonal partial least squares discriminant analysis, and a total of 19 aldehydes and ketones were quantified and 11 potential biomarkers were screened for the diagnosis of IAV infection.

Key words:

1. [1]
  GUÉRAUD F, ATALAY M, BRESGEN N, CIPAK A, ECKL P M, HUC L, JOUANIN I, SIEMS W, UCHIDA K. Free Radical Res., 2010, 44(10): 1098-1124.GUÉRAUD F, ATALAY M, BRESGEN N, CIPAK A, ECKL P M, HUC L, JOUANIN I, SIEMS W, UCHIDA K. Free Radical Res., 2010, 44(10): 1098-1124.
2. [2]
  LIU M, CHEN F, LIU T, CHEN F, LIU S, YANG J. Microbes Infect., 2017, 19(12): 580-586.LIU M, CHEN F, LIU T, CHEN F, LIU S, YANG J. Microbes Infect., 2017, 19(12): 580-586.
3. [3]
  TOYOKUNI S. IUBMB Life, 2008, 60(7): 441-447.TOYOKUNI S. IUBMB Life, 2008, 60(7): 441-447.
4. [4]
  MAZZONE P J. J. Thorac. Oncol., 2008, 3(7): 774-780.MAZZONE P J. J. Thorac. Oncol., 2008, 3(7): 774-780.
5. [5]
  MIEKISCH W, SCHUBERT J K, NOELDGE-SCHOMBURG G F E. Clin. Chim. Acta, 2004, 347(1-2): 25-39.MIEKISCH W, SCHUBERT J K, NOELDGE-SCHOMBURG G F E. Clin. Chim. Acta, 2004, 347(1-2): 25-39.
6. [6]
  POLI D, GOLDONI M, CORRADI M, ACAMPA O, CARBOGNANI P, INTERNULLO E, CASALINI A, MUTTI A. J. Chromatogr. B, 2010, 878(27): 2643-2651.POLI D, GOLDONI M, CORRADI M, ACAMPA O, CARBOGNANI P, INTERNULLO E, CASALINI A, MUTTI A. J. Chromatogr. B, 2010, 878(27): 2643-2651.
7. [7]
  TRAXLER S, BISCHOFF A C, SASS R, TREFZ P, GIERSCHNER P, BROCK B, SCHWAIGER T, KARTE C, BLOHM U, SCHRÖDER C, MIEKISCH W, SCHUBERT J K. Sci. Rep., 2018, 8: 14857.TRAXLER S, BISCHOFF A C, SASS R, TREFZ P, GIERSCHNER P, BROCK B, SCHWAIGER T, KARTE C, BLOHM U, SCHRÖDER C, MIEKISCH W, SCHUBERT J K. Sci. Rep., 2018, 8: 14857.
8. [8]
  SCHMIDT K, PODMORE I. J.Biomarkers, 2015, 2015: 981458.SCHMIDT K, PODMORE I. J.Biomarkers, 2015, 2015: 981458.
9. [9]
  FILIPIAK W, SPONRING A, FILIPIAK A, AGER C, SCHUBERT J, MIEKISCH W, AMANN A, TROPPMAIR J. Cancer Epidemiol., Biomarkers Prev., 2010, 19(1): 182-195.FILIPIAK W, SPONRING A, FILIPIAK A, AGER C, SCHUBERT J, MIEKISCH W, AMANN A, TROPPMAIR J. Cancer Epidemiol., Biomarkers Prev., 2010, 19(1): 182-195.
10. [10]
  TRAXLER S, BARKOWSKY G, SASS R, KLEMENZ A C, PATENGE N, KREIKEMEYER B, SCHUBERT J K, MIEKISCH W. Sci. Rep., 2019, 9: 18894.TRAXLER S, BARKOWSKY G, SASS R, KLEMENZ A C, PATENGE N, KREIKEMEYER B, SCHUBERT J K, MIEKISCH W. Sci. Rep., 2019, 9: 18894.
11. [11]
  BARANSKA A, SMOLINSKA A, BOOTSA W, DALLINGA J W, VAN SCHOOTEN F J. J. Breath Res., 2015, 9(4): 047102.BARANSKA A, SMOLINSKA A, BOOTSA W, DALLINGA J W, VAN SCHOOTEN F J. J. Breath Res., 2015, 9(4): 047102.
12. [12]
  FILIPIAK W, FILIPIAK A, SPONRING A, SCHMID T, ZELGER B, AGER C, KLODZINSKA E, DENZ H, PIZZINI A, LUCCIARINI P, JAMNIG H, TROPPMAIR J, AMANN A. J. Breath Res., 2014, 8(2): 027111.FILIPIAK W, FILIPIAK A, SPONRING A, SCHMID T, ZELGER B, AGER C, KLODZINSKA E, DENZ H, PIZZINI A, LUCCIARINI P, JAMNIG H, TROPPMAIR J, AMANN A. J. Breath Res., 2014, 8(2): 027111.
13. [13]
  JIA Z, ZHANG H, ONG C N, PATRA A, LU Y, LIM C T, VENKATESAN T. ACS Omega, 2018, 3(5): 5131-5140.JIA Z, ZHANG H, ONG C N, PATRA A, LU Y, LIM C T, VENKATESAN T. ACS Omega, 2018, 3(5): 5131-5140.
14. [14]
  WANG Y, HU Y, WANG D, YU K, WANG L, ZOU Y, ZHAO C, ZHANG X, WANG P, YING K. Cancer Biomarkers, 2012, 11: 129-137.WANG Y, HU Y, WANG D, YU K, WANG L, ZOU Y, ZHAO C, ZHANG X, WANG P, YING K. Cancer Biomarkers, 2012, 11: 129-137.
15. [15]
  CHEN X, XU F, WANG Y, PAN Y, LU D, WANG P, YING K, CHEN E, ZHANG W. Cancer, 2007, 110(4): 835-844.CHEN X, XU F, WANG Y, PAN Y, LU D, WANG P, YING K, CHEN E, ZHANG W. Cancer, 2007, 110(4): 835-844.
16. [16]
  LIGOR T, LIGOR M, AMANN A, AGER C, BACHLER M, DZIEN A, BUSZEWSKI B. J. Breath Res., 2008, 2(4): 046006.LIGOR T, LIGOR M, AMANN A, AGER C, BACHLER M, DZIEN A, BUSZEWSKI B. J. Breath Res., 2008, 2(4): 046006.
17. [17]
  WEHINGER A, SCHMID A, MECHTCHERIAKOV S, LEDOCHOWSKI M, GRABMER C, GASTL G A, AMANN A. Int. J. Mass Spectrom., 2007, 265(1): 49-59.WEHINGER A, SCHMID A, MECHTCHERIAKOV S, LEDOCHOWSKI M, GRABMER C, GASTL G A, AMANN A. Int. J. Mass Spectrom., 2007, 265(1): 49-59.
18. [18]
  SMITH D, ŠPANĚL P. Mass Spectrom. Rev., 2005, 24(5): 661-700.SMITH D, ŠPANĚL P. Mass Spectrom. Rev., 2005, 24(5): 661-700.
19. [19]
  PAL R, KIM K H. Anal. Chim. Acta, 2008, 610(2): 289-296.PAL R, KIM K H. Anal. Chim. Acta, 2008, 610(2): 289-296.
20. [20]
  BUNKOED O, THAVARUNGKUL P, THAMMAKHET C, KANATHARANA P. J. Environ. Sci. Health, Part A: Toxic/Hazard. Subst. Environ. Eng., 2012, 47(2): 167-175.BUNKOED O, THAVARUNGKUL P, THAMMAKHET C, KANATHARANA P. J. Environ. Sci. Health, Part A: Toxic/Hazard. Subst. Environ. Eng., 2012, 47(2): 167-175.
21. [21]
  LIN Y, DUEKER S R, JONES A D, EBELER S E, CLIFFORD A J. Clin. chem., 1995, 41(7): 1028-1032.LIN Y, DUEKER S R, JONES A D, EBELER S E, CLIFFORD A J. Clin. chem., 1995, 41(7): 1028-1032.
22. [22]
  CHEN Y, LI Q, XIE Z, FU X A. J. Chromatogr. B, 2019, 1106-1107: 58-63.CHEN Y, LI Q, XIE Z, FU X A. J. Chromatogr. B, 2019, 1106-1107: 58-63.
23. [23]
  OCHS S D, FASCIOTTI M, BARRETO R P, DE FIGUEIREDO N G, ALBUQUERQUE F C, PONTES MASSA M C G P, GABARDO I, PEREIRA NETTO A D P. Talanta, 2010, 81(1): 521-529.OCHS S D, FASCIOTTI M, BARRETO R P, DE FIGUEIREDO N G, ALBUQUERQUE F C, PONTES MASSA M C G P, GABARDO I, PEREIRA NETTO A D P. Talanta, 2010, 81(1): 521-529.
24. [24]
  CRABB D W, MATSUMOTO M, CHANG D, YOU M. Proc. Nutr. Soc., 2004, 63(1): 49-63.CRABB D W, MATSUMOTO M, CHANG D, YOU M. Proc. Nutr. Soc., 2004, 63(1): 49-63.
25. [25]
  JELSKI W, SZMITKOWSKI M. Clin. Chim. Acta, 2008, 395(1): 1-5.JELSKI W, SZMITKOWSKI M. Clin. Chim. Acta, 2008, 395(1): 1-5.
26. [26]
  NORDMANN R, RIBIERE C, ROUACH H, BEAUGE F, GIUDICELLI Y, NORDMANN J. Life Sci., 1973, 13(7): 919-932.NORDMANN R, RIBIERE C, ROUACH H, BEAUGE F, GIUDICELLI Y, NORDMANN J. Life Sci., 1973, 13(7): 919-932.
27. [27]
  JANFAZA S, KHORSAND B, NIKKHAH M, ZAHIRI J. Biol. Methods Protoc., 2019, 4(1): bpz014.JANFAZA S, KHORSAND B, NIKKHAH M, ZAHIRI J. Biol. Methods Protoc., 2019, 4(1): bpz014.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

细胞顶空气中典型醛酮化合物定量分析方法研究

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

Corresponding author: LI Xue, tamylee@jnu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

细胞顶空气中典型醛酮化合物定量分析方法研究

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

Corresponding author: LI Xue, tamylee@jnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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