基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素

引用本文: 焦婷, 温昊翔, 李忠平. 基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素[J]. 分析化学, 2022, 50(2): 235-243. doi: 10.19756/j.issn.0253-3820.210770 shu

Citation: JIAO Ting, WEN Hao-Xiang, LI Zhong-Ping. Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters[J]. Chinese Journal of Analytical Chemistry, 2022, 50(2): 235-243. doi: 10.19756/j.issn.0253-3820.210770 shu

基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素

焦婷 ^1, ,
温昊翔 ^3, ,
李忠平 ^{2,
,}

1.
山西大学化学化工学院, 太原 030006;
2.
山西大学环境科学研究所, 太原 030006;
3.
太原理工大学化学化工学院, 太原 030024

通讯作者: 李忠平,E-mail:zl104@sxu.edu.cn

基金项目:
国家自然科学基金项目（No.21575083）、晋中学院创新团队项目（No.jzxycxtd2019007）和第“十二批”山西省百人计划项目（No.128，129）资助

摘要: 以聚乙烯亚胺(PEI)为稳定剂，抗坏血酸(AA)为还原剂，制备了铜纳米簇(PEI@CuNCs)，并基于此建立了一种快速、简便的检测盐酸阿霉素(DOX)的方法。制备的PEI@CuNCs粒径约2.4 nm，最大激发波长为430 nm,最大发射波长为518 nm，具有良好的稳定性和抗光漂白性能。通过X射线光电子能谱以及傅里叶变换红外光谱等技术对PEI@CuNCs的表面官能团进行了表征。DOX可以猝灭PEI@CuNCs的荧光，因此将其作为荧光探针用于DOX的检测，在最优条件及1～145 μmol/L浓度范围内，DOX的浓度与F/F₀(F和F₀分别为存在和不存在DOX时探针的荧光强度)具有良好的线性关系，检出限(S/N=3)为0.032 μmol/L。DOX对探针的荧光猝灭机制为内滤效应(IFE)，本方法对DOX具有较高的选择性。血清中DOX的加标回收率为95.6%~105.5%，表明本方法具有良好的实用性。本研究为检测DOX提供了一种新方法。

关键词:

English

Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters

1.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
2.
Institute of Environmental Sciences, Shanxi University, Taiyuan 030006, China;
3.
School of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China

Corresponding author: LI Zhong-Ping, zl104@sxu.edu.cn

Received Date: 29 September 2021
Revised Date: 03 December 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.21575083), the Program for the Innovative Teams of Jinzhong University (No.jzxycxtd2019007) and the Hundred Talents Program of the Twelfth Batch Shanxi Province (No.128,129)

Abstract: A kind of novel copper nanoclusters (PEI@CuNCs) with sizes of about 2.4 nm was prepared using polyethyleneimine (PEI) as stabilizer and ascorbic acid as reducing agent, which could be used as probe to detect doxorubicin hydrochloride (DOX). The prepared PEI@CuNCs showed good stability and resistance to photobleaching, and their maximal excitation and emission wavelengths were 430 and 518 nm, respectively. The surface functional groups of PEI@CuNCs were characterized by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. DOX could quench the fluorescence of PEI@CuNCs, on the basis of this, a fluorescence method for detection of DOX was established. Under the optimal conditions, in the DOX concentration range of 1-145 μmol/L, a good linear relationship between the concentration of DOX and F/F₀ was observed, with a detection limit of 0.032 μmol/L (S/N=3). In the presence of other antibiotics, this method had high selectivity to DOX. The fluorescence quenching of PEI@CuNCs by DOX was attributed to internal filtration effect (IFE). This work showed great prospects for detecting DOX.

Key words:

1. [1]
  XIONG M H, BAO Y, DU X J, TAN Z B, JIANG Q, WANG H X, ZHU Y H, WANG J. ACS Nano, 2013,7(12): 10636-10645.XIONG M H, BAO Y, DU X J, TAN Z B, JIANG Q, WANG H X, ZHU Y H, WANG J. ACS Nano, 2013,7(12): 10636-10645.
2. [2]
  PARASTOO M A, MOHAMMAD H, JAFAR S, JALIL V G, ABOLGHASEM J. Microchem. J., 2019, 145: 450-455.PARASTOO M A, MOHAMMAD H, JAFAR S, JALIL V G, ABOLGHASEM J. Microchem. J., 2019, 145: 450-455.
3. [3]
  KIM Y A, CHO H, LEE N, JUNG S Y, SIM S H, PARK I H, LEE S, LEE E S, KIMH J. Cancer Med., 2018, 7(12): 6084-6092.KIM Y A, CHO H, LEE N, JUNG S Y, SIM S H, PARK I H, LEE S, LEE E S, KIMH J. Cancer Med., 2018, 7(12): 6084-6092.
4. [4]
  WANG M F, LIN J, GONG J W, MA M C, TANG H L, LIU J Y, YAN F. RSC Adv., 2021, 11(15): 9021-9028.WANG M F, LIN J, GONG J W, MA M C, TANG H L, LIU J Y, YAN F. RSC Adv., 2021, 11(15): 9021-9028.
5. [5]
  ROBIN D F, JENNIFER J G, JULIE D, ROBERT D C. J. Chromatogr. B, 2007, 852(1-2): 545-553.ROBIN D F, JENNIFER J G, JULIE D, ROBERT D C. J. Chromatogr. B, 2007, 852(1-2): 545-553.
6. [6]
  HU J, ZHAN S, WU X, HU S, WU S, LIU Y. RSC Adv., 2018, 8(38): 21505-21512.HU J, ZHAN S, WU X, HU S, WU S, LIU Y. RSC Adv., 2018, 8(38): 21505-21512.
7. [7]
  XU Z F, DENG P H, LI J H, XU L, TANG S P. Mater. Sci. Eng. B, 2017, 218: 31-39.XU Z F, DENG P H, LI J H, XU L, TANG S P. Mater. Sci. Eng. B, 2017, 218: 31-39.
8. [8]
  YANG M, YAN Y, LIU E, HU X, HAO H, FAN J. Optical Mater., 2021, 112: 1-6.YANG M, YAN Y, LIU E, HU X, HAO H, FAN J. Optical Mater., 2021, 112: 1-6.
9. [9]
  SHU T, WANG J X, LI X Q, ZHANG X J, SU L. Curr. Nanosci., 2015, 11(6): 702-709.SHU T, WANG J X, LI X Q, ZHANG X J, SU L. Curr. Nanosci., 2015, 11(6): 702-709.
10. [10]
  WANG S, LIU P, QIN Y, CHEN Z, SHEN J. Sens. Actuators, B, 2016, 223: 178-185.WANG S, LIU P, QIN Y, CHEN Z, SHEN J. Sens. Actuators, B, 2016, 223: 178-185.
11. [11]
  ZHANG B Z, WEI C Y. Anal. Bioanal. Chem., 2020, 412(11): 2529-2536.ZHANG B Z, WEI C Y. Anal. Bioanal. Chem., 2020, 412(11): 2529-2536.
12. [12]
  ZHOU M M, JIN S, WEI X, YUAN Q Q, WANG S X, DU Y X, ZHU M Z. J. Phys. Chem. C, 2020,124(13): 7531-7538.ZHOU M M, JIN S, WEI X, YUAN Q Q, WANG S X, DU Y X, ZHU M Z. J. Phys. Chem. C, 2020,124(13): 7531-7538.
13. [13]
  TAN N D, YIN J H, YUAN Y Q, MENG L, XU N. Bull. Korean Chem. Soc., 2018, 39(5): 657-664.TAN N D, YIN J H, YUAN Y Q, MENG L, XU N. Bull. Korean Chem. Soc., 2018, 39(5): 657-664.
14. [14]
  TAO Y, LI Z H, JU E G, REN J S, QU X G. Nanoscale, 2013, 5(13): 6154-6160.TAO Y, LI Z H, JU E G, REN J S, QU X G. Nanoscale, 2013, 5(13): 6154-6160.
15. [15]
  LIU Meng-Xuan, YIN Jian-Hang, MENG Lei, XU Na. Chin. J. Anal. Chem., 2020, 48(11): 1556-1563. 刘梦旋, 尹建行, 孟磊, 徐娜. 分析化学, 2020, 48(11): 1556-1563.
16. [16]
  SHEN Y M, TIAN R, MA H Y, SUN X H. Luminescence, 2021, 36(3): 705-710.SHEN Y M, TIAN R, MA H Y, SUN X H. Luminescence, 2021, 36(3): 705-710.
17. [17]
  REYES-CRUZALEY A P, OCHOA-TERAN A, TIRADO-GUIZAR A, FELIX-NAVARRO R M, ALONSO-NUNEZ G, PINA-LUIS G. Anal. Methods, 2021, 13(22): 2495-2503.REYES-CRUZALEY A P, OCHOA-TERAN A, TIRADO-GUIZAR A, FELIX-NAVARRO R M, ALONSO-NUNEZ G, PINA-LUIS G. Anal. Methods, 2021, 13(22): 2495-2503.
18. [18]
  ZHANG S, LI J, HUANG S, MA X, ZHANG C. Chem. Pap., 2021, 75(8): 3761-3769.ZHANG S, LI J, HUANG S, MA X, ZHANG C. Chem. Pap., 2021, 75(8): 3761-3769.
19. [19]
  LING Y, WU J J, GAO Z F, LI N B, LUO H Q. J. Phys. Chem. C, 2015, 119(48): 27173-27177.LING Y, WU J J, GAO Z F, LI N B, LUO H Q. J. Phys. Chem. C, 2015, 119(48): 27173-27177.
20. [20]
  SHANG L, DONG S J, NIENHAUS G U. Nano Today, 2011, 6(4): 401-418.SHANG L, DONG S J, NIENHAUS G U. Nano Today, 2011, 6(4): 401-418.
21. [21]
  DENG H H, LI K L, ZHUANG Q Q, PENG H P, ZHUANG Q Q, LIU A L, XIA X H, CHEN W. Nanoscale, 2018, 10(14): 6467-6473.DENG H H, LI K L, ZHUANG Q Q, PENG H P, ZHUANG Q Q, LIU A L, XIA X H, CHEN W. Nanoscale, 2018, 10(14): 6467-6473.
22. [22]
  ZHONG Y P, WANG Q P, HE Y, GE Y L, SONG G W. Sens. Actuators, B, 2015, 209: 147-153.ZHONG Y P, WANG Q P, HE Y, GE Y L, SONG G W. Sens. Actuators, B, 2015, 209: 147-153.
23. [23]
  JIA X, LI J, WANG E. Small, 2013, 9(22): 3873-3879.JIA X, LI J, WANG E. Small, 2013, 9(22): 3873-3879.
24. [24]
  CAI Z, ZHANG C, JIA K. Chem. Pap., 2020, 74(6): 1831-1838.CAI Z, ZHANG C, JIA K. Chem. Pap., 2020, 74(6): 1831-1838.
25. [25]
  FENG J, JU Y Y, LIU J J, ZHANG H G, CHEN X G. Anal. Chim. Acta, 2015, 854: 153-160.FENG J, JU Y Y, LIU J J, ZHANG H G, CHEN X G. Anal. Chim. Acta, 2015, 854: 153-160.
26. [26]
  WEN T, QU F, LI N B, LUO H Q. Anal. Chim. Acta, 2012, 749: 56-62.WEN T, QU F, LI N B, LUO H Q. Anal. Chim. Acta, 2012, 749: 56-62.
27. [27]
  MI G, SHI H, YANG M, WANG C, HAO H, FAN J. Spectrochim. Acta, Part A, 2020, 241: 1-7.MI G, SHI H, YANG M, WANG C, HAO H, FAN J. Spectrochim. Acta, Part A, 2020, 241: 1-7.
28. [28]
  HUANG K Y, HE H X, HE S B, ZHANG X P, PENG H P, LIN Z, DENG H H, XIA X H, CHEN W. Sens. Actuators, B, 2019, 296: 1-7.HUANG K Y, HE H X, HE S B, ZHANG X P, PENG H P, LIN Z, DENG H H, XIA X H, CHEN W. Sens. Actuators, B, 2019, 296: 1-7.
29. [29]
  CHEKIN F, MYSHIN V, YE R, MELINTE S, SINGH S K, KURUNGTO S, BOUKHERROUB R, SZUNERITS S. Anal. Bioanal. Chem., 2019, 411(8): 1509-1516.CHEKIN F, MYSHIN V, YE R, MELINTE S, SINGH S K, KURUNGTO S, BOUKHERROUB R, SZUNERITS S. Anal. Bioanal. Chem., 2019, 411(8): 1509-1516.
30. [30]
  ER E, ERK N. Microchim. Acta, 2020, 187(4): 1-9.ER E, ERK N. Microchim. Acta, 2020, 187(4): 1-9.
31. [31]
  MADRAKIAN T, ASL K D, AHMADI M, AFKHAMI A. RSC Adv., 2016, 6(76): 72803-72809.MADRAKIAN T, ASL K D, AHMADI M, AFKHAMI A. RSC Adv., 2016, 6(76): 72803-72809.
32. [32]
  YU J B, JIN H, GUI R J, WANG Z H, GE F. Talanta, 2017, 162: 435-439.YU J B, JIN H, GUI R J, WANG Z H, GE F. Talanta, 2017, 162: 435-439.
33. [33]
  YANG T Q, PENG B, Shan B Q, ZONG Y X, JIANG J G, WU P, ZHANG K. Nanomaterials, 2020, 10(2): 1-24.YANG T Q, PENG B, Shan B Q, ZONG Y X, JIANG J G, WU P, ZHANG K. Nanomaterials, 2020, 10(2): 1-24.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素

通讯作者: 李忠平,E-mail:zl104@sxu.edu.cn

English

Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters

Corresponding author: LI Zhong-Ping, zl104@sxu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素

通讯作者: 李忠平,E-mail:zl104@sxu.edu.cn

English

Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters

Corresponding author: LI Zhong-Ping, zl104@sxu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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