高效液相色谱-四极杆/飞行时间质谱法测定粮谷中茚嗪氟草胺及其代谢物

引用本文: 张嘉俊, 邓伟恒, 区硕俊, 王志元, 岑建斌, 陈敏丹, 王思威, 曾广丰. 高效液相色谱-四极杆/飞行时间质谱法测定粮谷中茚嗪氟草胺及其代谢物[J]. 分析化学, 2021, 49(8): 1366-1374. doi: 10.19756/j.issn.0253-3820.201403 shu

Citation: ZHANG Jia-Jun, DENG Wei-Heng, OU Shuo-Jun, WANG Zhi-Yuan, CEN Jian-Bin, CHEN Min-Dan, WANG Si-Wei, ZENG Guang-Feng. High Performance Liquid Chromatography-Tandem Quadrupole-Time of Flight-Mass Spectrometry for Determination of Indaziflam and Four Metabolites in Cereals[J]. Chinese Journal of Analytical Chemistry, 2021, 49(8): 1366-1374. doi: 10.19756/j.issn.0253-3820.201403 shu

高效液相色谱-四极杆/飞行时间质谱法测定粮谷中茚嗪氟草胺及其代谢物

张嘉俊 ^1, ,
邓伟恒 ^2, ,
区硕俊 ^1,2, ,
王志元 ^1, ,
岑建斌 ^2,1, ,
陈敏丹 ^3, ,
王思威 ^{1,
,} ,
曾广丰 ^,

1.
广州海关技术中心, 广州 510623;
2.
广州检验检测认证集团有限公司, 广州 511447;
3.
广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640

通讯作者: 王思威,E-mail:344073564@qq.com; 曾广丰,E-mail:zgf621@126.com

基金项目:
国家农产品质量安全风险评估重大专项子课题-热带水果中农药残留风险评估项目（No.GJFP202002）和广东省农业科学院院长基金项目（No.201707）资助。

摘要: 建立了高效液相色谱-四极杆-飞行时间质谱（HPLC-Q-TOF/MS）同时测定粮谷中茚嗪氟草胺及其4种代谢物（1-氟乙基三嗪二胺、茚嗪氟草胺-乙酸、三嗪-茚满酮、茚嗪氟草胺-烯烃）残留量的方法。样品经乙腈提取、盐析离心分层后，取上清液进行净化。在优化的色谱及质谱条件下，采用ESI离子源正离子扫描方式进行数据采集。5种化合物在2.0~200 μg/L范围内呈良好线性关系，相关系数（R²）均大于0.9987，在10、20和100 μg/kg添加水平下，平均回收率为79.3%~108.0%，相对标准偏差为1.2%~6.0%。方法检出限为0.05~0.60 μg/kg，定量限为0.15~2.00 μg/kg。本方法具有操作简便、回收率好、灵敏度高、重现性好等优点，可用于快速分析测定粮谷中茚嗪氟草胺及其代谢物残留。

关键词:

English

High Performance Liquid Chromatography-Tandem Quadrupole-Time of Flight-Mass Spectrometry for Determination of Indaziflam and Four Metabolites in Cereals

1.
Guangzhou Customs Technology Center, Guangzhou 510623, China;
2.
Guangzhou Inspection and Certification Group Co. Ltd., Guangzhou 511447, China;
3.
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding author: WANG Si-Wei, ; ZENG Guang-Feng,

Received Date: 11 July 2020
Revised Date: 30 May 2021
Fund Project:
Supported by the Sub Project of National Agricultural Product Quality and Safety Risk Assessment Major Special Project-Pesticide Residue Risk Assessment in Tropical Fruits (No.GJFP202002) and the Foundation of the Dean of Guangdong Academy of Agricultural Sciences (No.201707).

Abstract: A high performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry (HPLC-Q-TOF/MS) method for determination of residues of indaziflam and its four metabolites (1-fluoroethyl triazinediamine, indaziflam-carboxylic acid, triazine-indanone and indaziflam-olefin) in cereal samples was developed. The samples were extracted by acetonitrile, and the supernatant was collected for purification after salting-out and centrifugation. Under the optimized chromatographic and mass spectrometric conditions, the data were collected by electrospray ionization (ESI) sources in positive ion mode. The calibration curves of five compounds showed good linearity in concentration range of 2.0-200 μg/L (R²>0.9987). The average recoveries were 79.3%-108.0% at three spiked levels (10, 20 and 100 μg/kg), while the relative standard deviations (RSD) were 1.2%-6.0%. The limits of detection and limits of quantification ranged from 0.05 to 0.60 μg/kg and 0.15 to 2.00 μg/kg, respectively. This method was a simple and efficient method with high sensitivity and precision, and had practical value for the rapid detection of residues of indaziflam and its four metabolites in cereals.

Key words:

High performance liquid chromatography-tandem quadrupole-time-of-flight-mass spectrometry
/ Cereals
/ Indaziflam
/ Metabolite

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

高效液相色谱-四极杆/飞行时间质谱法测定粮谷中茚嗪氟草胺及其代谢物

通讯作者: 王思威,E-mail:344073564@qq.com; 曾广丰,E-mail:zgf621@126.com

English

High Performance Liquid Chromatography-Tandem Quadrupole-Time of Flight-Mass Spectrometry for Determination of Indaziflam and Four Metabolites in Cereals

Corresponding author: WANG Si-Wei, ; ZENG Guang-Feng,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高效液相色谱-四极杆/飞行时间质谱法测定粮谷中茚嗪氟草胺及其代谢物

通讯作者: 王思威,E-mail:344073564@qq.com; 曾广丰,E-mail:zgf621@126.com

English

High Performance Liquid Chromatography-Tandem Quadrupole-Time of Flight-Mass Spectrometry for Determination of Indaziflam and Four Metabolites in Cereals

Corresponding author: WANG Si-Wei, ; ZENG Guang-Feng,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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