基于时间分辨免疫分析的冰毒检测微流控芯片

引用本文: 孙敬敬, 宋祺, 牟颖. 基于时间分辨免疫分析的冰毒检测微流控芯片[J]. 分析化学, 2018, 46(7): 1116-1121. doi: 10.11895/j.issn.0253-3820.181216 shu

Citation: SUN Jing-Jing, SONG Qi, MU Ying. Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1116-1121. doi: 10.11895/j.issn.0253-3820.181216 shu

基于时间分辨免疫分析的冰毒检测微流控芯片

浙江大学智能系统与控制研究所, 工业控制技术国家重点实验室, 浙江大学分析仪器研究中心, 杭州 310027
基金项目:
本文系国家自然科学基金项目（No.81741145）及工业控制技术国家重点实验室开放课题（No.ICT1800381）资助

摘要: 研制了一种聚二甲基硅氧烷（Polydimethylsiloxane，PDMS）-多聚赖氨酸（Poly-l-lysine，PLL）玻片杂合微流控芯片，每张芯片有24条反应通道，每条反应通道体积仅为2.5 μL，实现了基于时间分辨免疫分析技术的高通量冰毒（Methamphetamine，MET）定量检测。此芯片利用PDMS和PLL对蛋白质的吸附特性在反应通道内表面固定冰毒完全抗原（MET-BSA），使其与待测样品中的冰毒抗原（MET-Ag）共同竞争标记有冰毒抗体（MET-Ab）的乳胶微球。乳胶微球表面标记了镧系元素，在紫外光的照射下会发出红色荧光，根据竞争法检测原理，冰毒浓度越高，与反应通道内表面冰毒完全抗原结合的乳胶微球数量越少，所发出荧光强度越低。本芯片的结果读取采用紫外照射荧光成像方法，仅需对芯片拍摄荧光图像，即可实现24个样本的同时检测分析。本方法样品消耗量少，通量大，可以在100~3000 ng/mL浓度范围内实现对冰毒的定量检测，可用于缉毒的初步筛查，具有较好的应用前景。

关键词:

English

Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay

Zhejiang University, Institute of Cyber-System and Control, State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Hangzhou 310027, China
Received Date: 31 March 2018
Revised Date: 02 May 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 81741145) and the Open Research Project of the State Key Laboratory of Industrial Control Technology, Zhejiang University, China (No. ICT1800381).

Abstract: A novel polydimethylsiloxane (PDMS)-poly-L-lysine (PLL) microfluidic chip, comprised of 24 reaction channels with 2.5 μL volume of each reaction channel only, was proposed for quantitative analysis of methamphetamine (MET) based on time-resolved immunoassay techniques. The chip utilized the adsorption characteristics of PDMS and PLL towards proteins to immobilize MET complete antigens (MET-BSA) on the surface of reaction channel. Then the competition reaction could happen between MET-Ag in the sample solution and MET-BSA on the inner surfaces of the reaction channels with MET-Ab in the reagent. The surface of latex microspheres was labeled by lanthanide, which could emit red fluorescence under the exposure of ultraviolet (UV). Based on the principle of competitive immunoassay, the more MET-Ag, the less the fluorescence intensity in the reaction channel. The detection results of this chip were acquired using UV-irradiation fluorescence imaging method. With this method, 24 samples could be detected and analyzed simultaneously on a chip by just taking the fluorescence image of the chip. The method allows the detection of MET antigens ranging from 100 ng/mL to 3000 ng/mL, with less sample consumption and high-throughput. This chip is suitable for the police preliminary screening work and has a good application prospects.

Key words:

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基于时间分辨免疫分析的冰毒检测微流控芯片

English

Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于时间分辨免疫分析的冰毒检测微流控芯片

English

Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

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