基于双极性离子阱内质子转移反应的多肽离子电荷调控方法研究

引用本文: 叶似剑, 龚晓云, 李明, 刘大军, 江游, 方向. 基于双极性离子阱内质子转移反应的多肽离子电荷调控方法研究[J]. 分析化学, 2018, 46(7): 1084-1088. doi: 10.11895/j.issn.0253-3820.171422 shu

Citation: YE Si-Jian, GONG Xiao-Yun, LI Ming, LIU Da-Jun, JIANG You, FANG Xiang. Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1084-1088. doi: 10.11895/j.issn.0253-3820.171422 shu

基于双极性离子阱内质子转移反应的多肽离子电荷调控方法研究

叶似剑 ^1, ,
龚晓云 ^2, ,
李明 ^2, ,
刘大军 ^1, ,
江游 ^2, ,
方向 ^2,

1.
长春理工大学, 化学与环境工程学院, 长春 130022;
2.
中国计量科学研究院, 北京 100029
基金项目:
本文系国家重点研发计划项目（No.2016YFF0200504）和国家自然科学基金项目（No.21605135）资助

摘要: 电喷雾是质谱分析多肽和蛋白质等生物分子时最常用的离子化技术。电喷雾产生的多肽和蛋白质离子通常带有多电荷，从而产生多个谱峰，导致谱图复杂。通过气相离子/离子间的质子转移反应可以有效调节经电喷雾离子化后蛋白和多肽等生物大分子的电荷价态，简化谱图，对于复杂蛋白和多肽生物样品的分析具有重要意义。本研究通过研制的一套双极性离子阱质谱装置，利用阱内正、负离子间的质子转移反应，实现了多肽离子的电荷调控。以氧化型谷胱甘肽、缩宫素、强啡肽分子作为典型样品对方法的检测效果进行了验证。结果表明，此方法可去除多肽正离子中多余的正电荷，当注入足够的负离子进行反应时，可以将带多个电荷的多肽阳离子的价态降至最低，从而有效简化谱图。

关键词:

English

Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry

1.
Changchun University of Science and Technology, Department of Chemistry & Environmental Engineering, Changchun 130022, China;
2.
National Institute of Metrology, Beijing 100029, China
Received Date: 13 November 2017
Revised Date: 10 May 2018
Fund Project:
This work was supported by the National Key Research and Development Program of China (No. 2016YFF0200504) and the National Natural Science Foundation of China (No. 21605135).

Abstract: Electrospray ionization is most commonly used in mass spectrometry for analysis of biological molecules such as peptides and proteins. However, peptides and proteins ions produced by electrospray ionization usually have multiple charges, and produce multiple spectrum peaks, making the mass spectrum complicated. Gas phase proton transfer ion/ion reaction can effectively regulate the charge states of peptides and proteins ions after electrospray ionization, simplifying the spectrogram, and thus is significant to the analysis of complex proteins and peptides samples. In this study, a dual-polarity linear ion trap (LIT) mass spectrometer was used to control the charge state of peptide ions by proton transfer ion/ion reaction. The detection effect of the method was verified by using glutathione (oxidation type), oxytocin and dynorphin as typical samples. The results showed that this method could remove excess charges in the positive ions. When injecting enough negative ions for eaction, peptide ions with multiple charge valence state could be reduced to the minimum, therefore simplify the spectrogram effectively.

Key words:

Proton transfer reaction
/ Peptide
/ Mass spectrometry
/ Positive and negative ion/ion reaction

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基于双极性离子阱内质子转移反应的多肽离子电荷调控方法研究

English

Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于双极性离子阱内质子转移反应的多肽离子电荷调控方法研究

English

Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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