功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究

引用本文: 何新宇, 王冰, 周洋洋, 卞晓军, 颜娟. 功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究[J]. 分析化学, 2018, 46(7): 1069-1076. doi: 10.11895/j.issn.0253-3820.171285 shu

Citation: HE Xin-Yu, WANG Bing, ZHOU Yang-Yang, BIAN Xiao-Jun, YAN Juan. Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1069-1076. doi: 10.11895/j.issn.0253-3820.171285 shu

功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究

1.
上海海洋大学食品学院, 上海 201306;
2.
上海水产品加工及贮藏工程技术研究中心, 上海 201306;
3.
农业部水产品贮藏保鲜质量安全风险评估实验室(上海), 上海 201306
基金项目:
本文系国家自然科学基金项目（Nos.21775102，21405167）和海洋工程国家重点实验室（上海交通大学）开放课题项目（No.1609）资助

摘要: 金属硫蛋白（Metallothionein，MT）是一种具有结合金属能力和高诱导特性的低分子量蛋白质，因此常被作为一种重要的生物标志物用于水环境重金属污染评估。传统的金属硫蛋白富集检测方法耗时较长，操作复杂。本研究制备了金包覆四氧化三铁的核壳纳米粒子（Fe₃O₄@Au NPs），具有磁场的快速响应和特殊的光学特性的优点，并且可利用Au-S的强亲和力实现纳米粒子对金属硫蛋白的快速富集，进一步将纯化的蛋白质通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）进行分析检测。结果表明，Fe₃O₄@Au纳米粒子可以直接从复杂的溶液中富集MT，采用MALDI-TOF/MS鉴定分析，检出限达10 fg/mL。

关键词:

English

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai), Ministry of Agriculture, Shanghai 201306, China
Received Date: 01 October 2017
Revised Date: 27 February 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21775102, 21405167), and the State Key Laboratory of Ocean Engineering (Shanghai Jiao Tong University), China (No. 1609).

Abstract: Metallothionein (MT) is a low-molecular-weight protein with high inducibility and binding ability with metal ions. Therefore, MT is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. But the traditional process of its enrichment and identification is time-consuming and complicated. Herein, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles (Fe₃O₄@Au NPs). The nanoparticle possessed the advantages such as fast response to magnetic fields and optical properties attributing to Fe₃O₄ and Au nanoparticles separately. Fe₃O₄@Au nanoparticles were used to enrich MT simply through AuS interaction, and the purified proteins were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The results in this work showed that the Fe₃O₄@Au nanoparticles could directly enrich MT from complex solutions and the detection limit could be down to 10 fg/mL.

Key words:

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1.
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功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究

English

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究

English

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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