竞争法免疫层析试条的数学模型与仿真研究

引用本文: 曾念寅, 朱盼盼, 李玉榕, 姜海燕, 杜民. 竞争法免疫层析试条的数学模型与仿真研究[J]. 分析化学, 2017, 45(9): 1284-1290. doi: 10.11895/j.issn.0253-3820.170281 shu

Citation: ZENG Nian-Yin, ZHU Pan-Pan, LI Yu-Rong, JIANG Hai-Yan, DU Min. Study on Mathematical Model and Simulation of Competition-type Lateral Flow Immunoassay[J]. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1284-1290. doi: 10.11895/j.issn.0253-3820.170281 shu

竞争法免疫层析试条的数学模型与仿真研究

1.
厦门大学仪器与电气系, 厦门 361005;2 福州大学福建省医疗器械和医药技术重点实验室, 福州 350116
基金项目:
本文系中国工程院与英国皇家工程院中英校企合作项目（No.UK-CIAPP\276）、国家自然科学基金（No.61403319）、福建省自然科学基金（No.2015J05131）和福建省生态产业绿色技术重点实验室开放课题项目资助

摘要: 根据竞争法免疫层析试条的检测原理，本研究将其分为TwA和TnA两种模式，结合对流扩散方程分别建立其数学模型，并运用COMSOL软件对试条的动态反应过程进行仿真，得到检测线和质控线上各复合物浓度关于各影响因素的关系曲线。在TwA模式中，分析了待测物浓度[A₀]=0-20 mol/L，标记物浓度[P₀]=0.01-100 mol/L，检测线位于5-20 mm位置等因素对于检测结果的影响；在TnA模式中，分析了[A₀]=0-20 mol/L，[P₀]=0.01-100 mol/L，[A₀]和[P₀]两种物质浓度及孔隙率等因素对于检测结果的影响。结果表明，本研究建立的竞争法模型与仿真能探究各参数对于检测结果的影响，优化试条性能，从而提高试条检测灵敏度和实现定量检测。

关键词:

English

Study on Mathematical Model and Simulation of Competition-type Lateral Flow Immunoassay

1.
Department of Instrumental and Electrical Engineering, Xiamen University, Xiamen 361005, China;2 Fujian Key Laboratory of Medical Instrumentation and Pharmaceutical Technology, Fuzhou University, Fuzhou 350116, China
Received Date: 04 May 2017
Revised Date: 21 July 2017
Fund Project:
This work was supported by the UK-China Industry Academia Partnership Programme (No.UK-CIAPP/276), the National Natural Science Foundation of China (No. 61403319), the Natural Science Foundation of Fujian Province, China (No. 2015J05131) and the Fujian Provincial Key Laboratory of Eco-Industrial Green Technology.

Abstract: A mathematical model of competition-type lateral flow immunoassay (LFIA) was developed to describe the dynamic process of LFIA. The competition-type LFIA was divided into two categories:TwA-competition-type LFIA and TnA-competition-type LFIA. On the basis of the developed model, the COMSOL software was exploited to simulate the dynamic process of LFIA. The simulation result demonstrated the relationships between the concentrations of substances on the test and control lines and the influence factors. In particular, the influence factors in the TwA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the position of the test line (5-20 mm). On the other hand, the influence factors in the TnA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the porosity. Experiment result showed that the developed model could be used to explore the influence of the parameters on the test results, and optimize the performance of LFIA.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

竞争法免疫层析试条的数学模型与仿真研究

English

Study on Mathematical Model and Simulation of Competition-type Lateral Flow Immunoassay

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

竞争法免疫层析试条的数学模型与仿真研究

English

Study on Mathematical Model and Simulation of Competition-type Lateral Flow Immunoassay

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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