基于抗原决定基的胰岛素分子印迹电化学传感器

引用本文: 赵成军, 马雄辉, 李建平. 基于抗原决定基的胰岛素分子印迹电化学传感器[J]. 分析化学, 2017, 45(9): 1360-1366. doi: 10.11895/j.issn.0253-3820.170256 shu

Citation: ZHAO Cheng-Jun, MA Xiong-Hui, Li Jian-Ping. An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting[J]. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1360-1366. doi: 10.11895/j.issn.0253-3820.170256 shu

基于抗原决定基的胰岛素分子印迹电化学传感器

广西电磁化学功能物质重点实验室, 桂林理工大学化学与生物工程学院, 桂林 541004
基金项目:
本文系国家自然科学基金（Nos.21375031，21765006）项目、广西自然科学基金创新研究团队项目（No.2015GXNSFFA139005）和广西高等学校高水平创新团队及卓越学者计划项目（桂教人[2014]49号）资助

摘要: 采用抗原决定基法制备了胰岛素电化学分子印迹传感器。以胰岛素C端多肽作为模板分子，定向自组装在Au电极上，以邻苯二胺为功能单体，电化学聚合制备分子印迹聚合膜。以NaOH为洗脱液，洗脱模板分子，形成的与胰岛素C端多肽三维结构相匹配的分子印迹孔穴能特异性识别胰岛素。重吸附胰岛素分子后，以K₃[Fe（CN）₆]/K₄[Fe（CN）₆]为探针，通过测量探针在电极表面产生的电流大小实现胰岛素的间接测定。在1.0×10^-14~5.0×10^-13 mol/L浓度范围内，传感器的电流响应值与胰岛素浓度呈良好的线性关系，检出限为7.24×10^-15 mol/L（3σ）。此传感器具有较好的选择性和稳定性，并成功用于血清样品中胰岛素的测定。

关键词:

English

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China
Received Date: 23 April 2017
Revised Date: 06 July 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21375031, 21765006) and the Natural Science Foundation of Guangxi Province, China (No. 2015GXNSFFA139005).

Abstract: A novel molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. C-Terminal polypeptide in insulin as template molecule was firstly self-assembled on the Au electrode. Then the molecularly imprinted polymer (MIP) was fabricated by electropolymerization with o-phenylenediamine (o-PD) as functional monomer on this Au electrode. After elution of template molecules by NaOH solution, the imprinting cavities were formed with the three-dimensional structure matched with the polypeptide in insulin molecules. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K₃[Fe(CN)₆]/K₄[Fe(CN)₆] as a probe, the insulin was indirectly detected. There was a linear relationship between the response current and the insulin concentrations in the range of 1.0×10^-14-5.0×10^-13 mol/L, and the detection limit was 7.24×10^-15 mol/L. The developed sensor exhibited good selectivity and stability, and could be applied to the determination of serum samples.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于抗原决定基的胰岛素分子印迹电化学传感器

English

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

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中国化学会秘书处

基于抗原决定基的胰岛素分子印迹电化学传感器

English

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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