非靶向尿液代谢组学方法研究五味子治疗糖尿病并发症作用机制

引用本文: 韩红祥, 宋凤瑞, 舒振波, 刘志强, 任跃英, 皮子凤. 非靶向尿液代谢组学方法研究五味子治疗糖尿病并发症作用机制[J]. 分析化学, 2017, 45(3): 389-396. doi: 10.11895/j.issn.0253-3820.160753 shu

Citation: HAN Hong-Xiang, SONG Feng-Rui, SHU Zhen-Bo, LIU Zhi-Qiang, REN Yue-Ying, PI Zi-Feng. An Untargeted Urinary Metabolomics Strategy for Investigation of Therapeutical Mechanism of Schisandra chinensis on Complications of Diabetes Rats[J]. Chinese Journal of Analytical Chemistry, 2017, 45(3): 389-396. doi: 10.11895/j.issn.0253-3820.160753 shu

非靶向尿液代谢组学方法研究五味子治疗糖尿病并发症作用机制

1.
吉林农业大学中药材学院, 长春 130118;
2.
中国科学院长春应用化学研究所, 长春质谱中心, 吉林省中药化学与质谱重点实验室, 长春 130022;
3.
吉林省现代中药及生物制药基地建设办公室, 长春 130041;
4.
吉林大学中日联谊医院, 长春 130033
基金项目:
本文系国家自然科学基金(Nos.81373952，81473537)资助项目

摘要: 采用超高效液相色谱-串联四级杆飞行时间质谱联用技术(Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry，UPLC/Q-TOF-MS)，结合主成分分析技术(Principal component analysis，PCA)，进行了五味子治疗糖尿病并发症大鼠尿液代谢组学研究，对五味子治疗糖尿病并发症大鼠后尿液生物标志物变化进行了考察。采用UPLC/Q-TOF-MS方法分析了健康组、糖尿病并发症模型组和五味子给药组的大鼠尿液，采用偏最小二乘法-判别分析(Partial least squares discriminant analysis，PLS-DA)和正交偏最小二乘法-判别分析(Orthogonal partial least squares discriminant analysis，OPLS-DA)载荷图筛选，通过分析对各组分离贡献较大(VIP>1，p<0.05)化合物的串联质谱数据，再经Human Metabolome Database(HMDB)等数据库检索，进行质谱信息匹配，尿液中共鉴定出28种潜在生物标记物，其中正谱13种，负谱15种。这些生物标记物主要影响戊糖和葡糖醛酸相互转化通路、核黄素代谢、泛酸和CoA合成、精氨酸和脯氨酸代谢、肠内菌代谢、嘌呤代谢、Vc代谢胆酸合成、色氨酸代谢等通路，五味子从能量代谢、氨基酸代谢、肠内菌代谢、脂类代谢等各个角度发挥治疗糖尿病并发症的作用。从各个通路的相关生物功能分析，五味子治疗糖尿病肾病的作用较强，此外还具有降脂、抗氧化的功效。

关键词:

English

An Untargeted Urinary Metabolomics Strategy for Investigation of Therapeutical Mechanism of Schisandra chinensis on Complications of Diabetes Rats

1.
Jilin Agricultural University, College of Chinese medicine, Changchun 130118, China;
2.
National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
3.
Office of TCM and Biotechnology Pharmacy of Jilin Province, Changchun 130041, China;
4.
China-Japan Union Hospital, Jilin University, Changchun 130033, China
Received Date: 12 October 2016
Revised Date: 21 November 2016
Fund Project:
The work was supported by the National Natural Science Foundation of China (Nos. 81373952, 81473537)

Abstract: An untargeted urinary metabonomics method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been established to investigate the mechanism of Schisandra chinensis in treating diabetes and its complications. The urinary biomarkers related to the therapeutic effects of Schisandra chinensis on the diabetes rats were analyzed. In urine, 28 kinds of endogenous metabolites were identified as potential biomarkers, including 13 endogenous metabolites in positive ion mode, 15 endogenous metabolites in negative ion mode, and hippuric acid detected both in positive and negative ion modes. The results revealed that Schisandra chinensis mainly affected the pathways of pentose and glucuronate interconversions, riboflavin metabolism, pantothenate and CoA biosynthesis, arginine and proline metabolism, intestinal bacteria metabolism, ascorbate and aldarate metabolism and tryptophan metabolism in diabetic rats. Combined with biological analysis of these pathways, the therapeutic mechanism of Schisandra chinensis on diabetes and its complications was verified. Based on the biological function of each pathway, the effect of Schisandra chinensis on diabetic nephropathy is stronger. Moreover, it also has the effects of protecting liver, decreasing fat and antioxidant activity.

Key words:

Untargeted metabolomics
/ Schisandra chinensis
/ Diabetes mellitus and its complications
/ Mechanism

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沈阳化工大学材料科学与工程学院沈阳 110142

非靶向尿液代谢组学方法研究五味子治疗糖尿病并发症作用机制

English

An Untargeted Urinary Metabolomics Strategy for Investigation of Therapeutical Mechanism of Schisandra chinensis on Complications of Diabetes Rats

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

非靶向尿液代谢组学方法研究五味子治疗糖尿病并发症作用机制

English

An Untargeted Urinary Metabolomics Strategy for Investigation of Therapeutical Mechanism of Schisandra chinensis on Complications of Diabetes Rats

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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