富镉离子CdS量子点电化学发光法检测<i>L</i>-半胱氨酸

引用本文: 姜瑞, 杨学梅, 王明丽, 杨敏丽. 富镉离子CdS量子点电化学发光法检测L-半胱氨酸[J]. 分析化学, 2017, 45(3): 409-415. doi: 10.11895/j.issn.0253-3820.160724 shu

Citation: JIANG Rui, YANG Xue-Mei, WANG Ming-Li, YANG Min-Li. Ultrasensitive Determination of L-Cysteine Based on Its Enhancement to Electrochemiluminescence of CdS Quantum Dots with Cd²⁺ Rich Surface[J]. Chinese Journal of Analytical Chemistry, 2017, 45(3): 409-415. doi: 10.11895/j.issn.0253-3820.160724 shu

富镉离子CdS量子点电化学发光法检测L-半胱氨酸

上海师范大学生命与环境科学学院, 上海 200234

摘要: 采用一锅法，通过控制镉硫比合成了表面富镉离子的硫化镉量子点，利用L-半胱氨酸可与量子点表面Cd²⁺结合，使量子点表面钝化，从而增强其电化学发光信号的性质，实现了对L-半胱氨酸的选择性检测。对合成的量子点进行了表征，优化了检测条件。在优化的条件下，L-半胱氨酸在5.0×10^-9-1.0×10^-5 mol/L浓度范围内与ECL信号呈良好的线性关系，检出限为1.2×10^-9 mol/L(S/N=3)。本方法对L-半胱氨酸具有良好的选择性，用于实际样品中L-半胱氨酸的测定，结果令人满意。

关键词:

English

Ultrasensitive Determination of L-Cysteine Based on Its Enhancement to Electrochemiluminescence of CdS Quantum Dots with Cd²⁺ Rich Surface

College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, China
Received Date: 27 September 2016
Revised Date: 11 January 2017

Abstract: A novel electrochemiluminescence (ECL) method for the determination of L-cysteine (L-Cys) was established. Water-soluble CdS quantum dots (QDs) with Cd²⁺ rich surface were synthesized via a controllable one-poe approach. The mercapto group in L-cysteine molecule can specifically interact with excessive Cd²⁺ on the surface of CdS QDs, resulting in enhancement of ECL intensity of the CdS QDs, which can be used for the detection of L-Cys. Under the optimal experimental conditions, the enhancement of ECL intensity was linear with the concentration of L-Cys in the range of 5.0×10^-9-1.0×10^-5 mol/L. The limit detection of (S/N=3) was 1.2×10^-9 mol/L. In comparison with other methods for detecting L-Cys, this method is more simple and selective, and can be applied to detect L-Cys in real sample with satisfactory results.

Key words:

Electrochemiluminescence
/ CdS quantum dots with Cd²⁺rich surface
/ L-Cysteine

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富镉离子CdS量子点电化学发光法检测L-半胱氨酸

English

Ultrasensitive Determination of L-Cysteine Based on Its Enhancement to Electrochemiluminescence of CdS Quantum Dots with Cd²⁺ Rich Surface

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

富镉离子CdS量子点电化学发光法检测L-半胱氨酸

English

Ultrasensitive Determination of L-Cysteine Based on Its Enhancement to Electrochemiluminescence of CdS Quantum Dots with Cd2+ Rich Surface

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Ultrasensitive Determination of L-Cysteine Based on Its Enhancement to Electrochemiluminescence of CdS Quantum Dots with Cd²⁺ Rich Surface

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs