基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析

引用本文: 刘晓莹, 马璐瑶, 王力, 汪星逸, 聂少平, 谢明勇, 殷军艺. 基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析[J]. 分析化学, 2017, 45(3): 416-422. doi: 10.11895/j.issn.0253-3820.160685 shu

Citation: LIU Xiao-Ying, MA Lu-Yao, WANG Li, WANG Xing-Yi, NIE Shao-Ping, XIE Ming-Yong, YIN Jun-Yi. Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection[J]. Chinese Journal of Analytical Chemistry, 2017, 45(3): 416-422. doi: 10.11895/j.issn.0253-3820.160685 shu

基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析

南昌大学食品科学与技术国家重点实验室, 南昌 330047
基金项目:
本文系国家自然科学基金项目(Nos.31301434，21564007)、江西省优势科技创新团队(No.20133BCB24001)，江西省自然科学基金(No.20142BAB214004)资助

摘要: 以大粒车前子来源阿拉伯木聚糖为研究对象，采用完全酸水解结合高效阴离子色谱-脉冲安培法测定单糖组成，系统优化酸的种类和浓度、水解温度和时间、水解后样品放置时间等水解条件。结果表明，将车前子多糖置于2 mol/L H₂SO₄、120℃常压油浴条件下水解2 h时，效果较好，但水解后稀释液放置时间不宜超过6 h。大粒车前子多糖主要由阿拉伯糖(8.89%)和木糖(41.52%)组成，同时检测出半乳糖醛酸(0.73%)、葡萄糖醛酸(3.44%)和微量的半乳糖、葡萄糖，结果重现性良好。利用上述最优条件水解黍子壳、燕麦麸皮和青稞来源阿拉伯木聚糖并分析单糖组成，均获得较好结果。本研究为分析各种来源阿拉伯木聚糖的单糖组成提供了参考。

关键词:

English

Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
Received Date: 12 September 2016
Revised Date: 16 December 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 31301434, 21564007), the Outstanding Science and Technology Innovation Team Project in Jiangxi Province (No. 20133BCB24001) and the Natural Science Foundation of Jiangxi Province, China (No. 20142BAB214004)

Abstract: A method of complete acid hydrolysis combined with high performance anion exchange chromatography and pulsed amperometric detection was developed for the monosaccharide composition analysis of arabinoxylan from the seeds of plantago asiatica L. The parameters including hydrolysis methods, acid types, acid concentration, hydrolysis temperature, hydrolysis time and placement time, which would affect the hydrolysis process, were optimized. The results showed that it would have a better hydrolysis effect for polysaccharide from the seeds of Plantago asiatica L. with 2 mol/L H₂SO₄ in an atmospheric oil bath at 120℃ for 2 hours. However, the placement time for diluted solution of the hydrolyzed polysaccharide should be less than 6 hours. The polysaccharide was mainly composed of Arabinose (8.89%) and Xylose (41.52%) and Galacturonic acid (0.73%). Glcuronic acid (3.44%) was detected simultaneously, and there were also trace amounts of Galatose and Glucose. The results were reproducible. Other arabinoxylans from Panicummiliaceum L. shell, Avena sativa L. bran and Hordeum vulgare L. were taken for monosaccharide compositions analysis under the optimal hydrolysis conditions and the analysis results were good. This study would provide a good reference for monosaccharides composition analysis of arabinoxylans from various sources.

Key words:

Arabinoxylan
/ Seeds from plantago asiatica L.
/ High performance anion exchange chromatography
/ Hydrolysis condition
/ Monosaccharide composition

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基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析

English

Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析

English

Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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