小儿肺炎患者尿液的代谢组学初步研究

引用本文: 李静, 刘晓蕾, 赵旭, 李巍, 戴雨霖, 越皓. 小儿肺炎患者尿液的代谢组学初步研究[J]. 分析化学, 2016, 44(3): 451-455. doi: 10.11895/j.issn.0253-3820.150872 shu

Citation: LI Jing, LIU Xiao-Lei, ZHAO Xu, LI Wei, DAI Yu-Lin, YUE Hao. A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 451-455. doi: 10.11895/j.issn.0253-3820.150872 shu

小儿肺炎患者尿液的代谢组学初步研究

李静 ^1, ,
刘晓蕾 ^2, ,
赵旭 ^3, ,
李巍 ^4, ,
戴雨霖 ^{2,
,} ,
越皓 ^{2,
,}

1.
¹ 长春中医药大学附属医院, 长春 130021;
2.
² 长春中医药大学吉林省人参科学研究院, 长春 130117;
3.
³ 吉林大学第一医院肝胆胰内科, 长春 130021;
4.
⁴ 深圳市第三人民医院, 深圳 518112

通讯作者: 戴雨霖, 越皓; 戴雨霖, 越皓

基金项目:
本文系公益性行业(农业)专项(No.20130311106)资助 (农业)专项(No.20130311106)

摘要: 利用液相色谱-质谱联用法对小儿肺炎(Childhood pneumonia, CP)患者和健康儿童(Healthy control)的尿液进行分析,发现小儿肺炎患者尿液中的潜在标记物,为其发病机制及早期筛查提供科学依据。筛选10例小儿肺炎患者(age 47.72±2.35 months)及10例健康儿童(age 46.65±1.97 months)尿液样本,采用快速高分辨液相色谱四极杆-飞行时间质谱联用(RRLC-Q TOF/MS)技术对其尿液代谢物进行分析,通过主成分分析方法(PCA)对两组代谢物进行分类,并发现潜在生物标记物。RRLC-Q TOF/MS检测表明,CP组和Healthy Control组尿液代谢物图谱能得到很好的区分,并鉴定了5种生物标记物,提示嘌呤代谢、氨基酸代谢可能在小儿肺炎发生发展中有重要作用。

关键词:

English

A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

LI Jing ^1, ,
LIU Xiao-Lei ^2, ,
ZHAO Xu ^3, ,
LI Wei ^4, ,
DAI Yu-Lin ^{2,
,} ,
YUE Hao ^{2,
,}

1.
¹ Hospital Affiliated to Changchun University of Traditional Chinese Medicine, Changchun 130021, China;
2.
² JiLin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China;
3.
³ The First Hosital of Jilin University, Changchun 130021, China;
4.
⁴ Shenzhen Third People's Hospital, Shenzhen 518112, China

Corresponding author: 戴雨霖, 越皓; 戴雨霖, 越皓

Received Date: 01 November 2015
Fund Project:
本文系公益性行业(农业)专项(No.20130311106)资助

Abstract: In this study, the rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from Childhood Pneumonia (CP) patients and healthy controls and find the potential biomarkers which can support evidence to early diagnose and cure the disease. Choose 10 CP patients (age 47.72±2.35 months) and 10 healthy controls (age 46.65±1.97 months). The urine samples were analyzed by RRLC-QTOF/MS and then the resulting data matrices were analyzed by principal components analysis (PCA) to find the potential biomarkers. Urine samples of CP patients were successfully distinguished from those of healthy controls. A total of two significantly changed metabolites have been found and identified as potential biomarkers. It is suggested that the disorder of purine metabolism and amino acid metabolism may play an important role in the mechanism of CP.

Key words:

Childhood Pneumonia
/ Rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry
/ Uric acid
/ Metabonomics

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

小儿肺炎患者尿液的代谢组学初步研究

通讯作者: 戴雨霖, 越皓; 戴雨霖, 越皓

English

A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

Corresponding author: 戴雨霖, 越皓; 戴雨霖, 越皓

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

小儿肺炎患者尿液的代谢组学初步研究

通讯作者: 戴雨霖, 越皓; 戴雨霖, 越皓

English

A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

Corresponding author: 戴雨霖, 越皓; 戴雨霖, 越皓

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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