全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素

引用本文: 夏群, 辛培勇, 褚金芳. 全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素[J]. 分析化学, 2016, 44(3): 409-415. doi: 10.11895/j.issn.0253-3820.150769 shu

Citation: XIA Qun, XIN Pei-Yong, CHU Jin-Fang. An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 409-415. doi: 10.11895/j.issn.0253-3820.150769 shu

全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素

夏群 ^1,2, ,
辛培勇 ^1, ,
褚金芳 ^{1,
,}

1.
¹ 中国科学院遗传与发育生物学研究所国家植物基因研究中心(北京), 北京 100101;
2.
² 中国科学院大学, 北京 100049

通讯作者: 褚金芳

基金项目:
本文系国家自然科学基金(No.91417314) (No.91417314)

中科院仪器设备功能开发技术创新项目和中科院关键技术人才项目资助

摘要: 建立了同时检测水稻中6种内源性植物激素脱落酸(Abscisic acid,ABA)、吲哚-3-乙酸(Indole-3-acetic acid, IAA)、水杨酸(Salicylic acid,SA)、茉莉酸(Jasmonic acid,JA)、吲哚-3-丙酸(Indole-3-propionic acid,IPA)和吲哚-3-丁酸(Indole-3-butyric acid,IBA)的全自动在线固相萃取-液相色谱-串联质谱方法。植物样品经过甲醇提取,采用C₁₈固相萃取柱富集净化,流动相将待测物洗脱至C₁₈分析色谱柱进行分离,最终使用串联四极杆质谱进行检测。方法的线性范围为8~320 μg/L,相关系数为R²≥0.99; 方法的检出限(S/N=3)范围为0.1~0.8 μg/kg; 实际样品中方法回收率范围为71.2%~126%,RSD<13%。应用本方法快速、准确地检测了水稻幼穗中多种内源性植物激素的含量,并与目前植物学领域内常用的检测方法进行了比较。同时,本方法对水稻受伤叶片的内源植物激素含量变化进行了定量分析,其含量随受伤时间的变化趋势与其生物背景的实验结果相吻合。

关键词:

English

An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice

1.
¹ National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
2.
² University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: 褚金芳

Received Date: 29 September 2015
Fund Project:
本文系国家自然科学基金(No.91417314)

中科院仪器设备功能开发技术创新项目和中科院关键技术人才项目资助

Abstract: An automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry (on-line SPE-LC-MS/MS) method for the simultaneous quantification of six endogenous phytohormones including abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA), indole-3-propionic acid (IPA) and indole-3-butyric acid (IBA) in rice tissues was described. Plant samples were extracted with methanol and on-line SPE procedure was performed with C₁₈ SPE cartridges. Analytes were eluted to C₁₈ analytical column with mobile phase and further analyzed by LC-MS/MS. The performance of the method was fully validated. Linearity showed good correlation (R²≥0.99). Limits of detections (S/N=3) were in the range of 0.1-0.8 μg／kg. Recoveries varied between 71.2% and 126%. The method was rapid and sensitive to determine multiple phytohormones in rice young panicles and the results were cross-validated with the off-line SPE-LC-MS/MS phytohormone analytical method. Finally, the method was applied to monitor the changes of ABA, IAA, SA and JA in wounded rice leaves. The trends were in accord with plant physiological processes.

Key words:

Phytohormones
/ On-line solid phase extraction-liquid chromatography-tandem mass spectrometry
/ Quantitative analysis
/ Rice

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素

通讯作者: 褚金芳

English

An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice

Corresponding author: 褚金芳

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素

通讯作者: 褚金芳

English

An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice

Corresponding author: 褚金芳

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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