基于壳聚糖/Ru(bpy)32+/SiO2纳米粒子电化学发光传感器用于尿酸的检测

引用本文: 樊雪梅, 王书民, 李哲建, 郑行望. 基于壳聚糖/Ru(bpy)₃²⁺/SiO₂纳米粒子电化学发光传感器用于尿酸的检测[J]. 分析化学, 2016, 44(3): 342-347. doi: 10.11895/j.issn.0253-3820.150705 shu

Citation: FAN Xue-Mei, WANG Shu-Min, LI Zhe-Jian, ZHENG Xing-Wang. Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 342-347. doi: 10.11895/j.issn.0253-3820.150705 shu

基于壳聚糖/Ru(bpy)₃²⁺/SiO₂纳米粒子电化学发光传感器用于尿酸的检测

樊雪梅 ^{1,
,} ,
王书民 ^1, ,
李哲建 ^1, ,
郑行望 ^2,

1.
¹ 商洛学院化学工程与现代材料学院, 商洛 726000;
2.
² 陕西师范大学化学化工学院, 西安 710062

通讯作者: 樊雪梅

基金项目:
本文系国家自然科学基金(No.30970696) (No.30970696)

陕西省科技统筹创新工程计划项目(2012KTDZ02-02) (2012KTDZ02-02)

商洛市科技局计划项目(SK-2014-4) (SK-2014-4)

陕西省尾矿资源综合利用重点实验室项目(14SKY-FWK06)资助。 (14SKY-FWK06)

摘要: 利用反相微乳液法制备了壳聚糖-Ru(bpy)₃²⁺-SiO₂复合纳米粒子,采用Nafion/MCNT复合膜技术实现了对复合纳米粒子有效而稳定的固定,从而制备了电化学发光传感器,实现了对尿酸的检测。在0.1 mol/L PBS缓冲溶液(pH 7.4)中,当尿酸与修饰电极作用15 min时,电化学发光强度与尿酸浓度(1.0×10^-10~1.0×10^-5 mol/L)的负对数呈良好的线性关系,线性方程为I_ECL=-709.52-202.74lgC,相关系数R=0.9936,检出限为6.0 × 10^-12 mol/L。传感器表现出良好的重现性与稳定性,对1.0 × 10^-8 mol/L尿酸平行测定11次,发光强度的相对标准偏差为2.9%,测定尿酸实际样品的加标回收率在 98.5%-103.5%之间。

关键词:

English

Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor

1.
¹ College of Chemical Engineering and Modern Materials, Shangluo University, Shangluo 726000, China;
2.
² School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China

Corresponding author: 樊雪梅

Received Date: 06 September 2015
Fund Project:
本文系国家自然科学基金(No.30970696)

陕西省科技统筹创新工程计划项目(2012KTDZ02-02)

商洛市科技局计划项目(SK-2014-4)

陕西省尾矿资源综合利用重点实验室项目(14SKY-FWK06)资助。

Abstract: Chitosan-Ru(bpy)₃²⁺-SiO₂ composite nanoparticles (CRuS NPs) were prepared by reverse microemulsion method, and based on the Nafion/MCNT composite membrane technology, CRuS NPs were effectively and steadily immobilize on the surface of a glassy carbon electrode to prepare the electrochemiluminescence sensor for uric acid determination. In 0.1 mol/L PBS (pH 7.4) buffer solution, when the actuation duration between uric acid and the modified electrode was 15 min, the electrochemiluminescence showed a good linear relationship to the negative logarithm of uric acid concentration in the range of 1.0 × 10^-10-1.0 × 10^-5 mol/L, the linear equation was I_ECL=-709.52-202.74lgC and the correlation coefficient was 0.9936 with a detection limit of 6.0 × 10^-12 mol/L. The ECL sensor exhibited excellent repeatability and stability, and the RSD for 11 times determination of 1.0 × 10^-8 mol/L uric acid was 2.9%. The recovery was 98.5%-103.5% in the determination of real Uric acid sample.

Key words:

Electrogenerated chemiluminescence
/ Chitosan-Ru(bpy)₃²⁺-SiO₂ nanoparticles
/ Uric acid

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于壳聚糖/Ru(bpy)₃²⁺/SiO₂纳米粒子电化学发光传感器用于尿酸的检测

通讯作者: 樊雪梅

English

Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor

Corresponding author: 樊雪梅

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