2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙-Cu(Ⅱ)-CopC三元配合物的光谱研究

引用本文: 任小林, 宋珍, 杨斌盛. 2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙-Cu(Ⅱ)-CopC三元配合物的光谱研究[J]. 无机化学学报, 2015, 31(9): 1811-1819. doi: 10.11862/CJIC.2015.244 shu

Citation: REN Xiao-Lin, SONG Zhen, YANG Bin-Sheng. Spectral Studies on Complex of 2,6-Pyridine Diformylhydrazine 2-Hydroxylnaphthene Carboxylic Hydrazone-Cu(Ⅱ)-CopC[J]. Chinese Journal of Inorganic Chemistry, 2015, 31(9): 1811-1819. doi: 10.11862/CJIC.2015.244 shu

2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙-Cu(Ⅱ)-CopC三元配合物的光谱研究

1.
山西大学分子科学研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006

通讯作者: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

基金项目:
国家自然科学基金(No.20771068,20901048) (No.20771068,20901048)

高等学校博士学科点专项科研基金(No.20131401110011)资助项目。 (No.20131401110011)

摘要: 以2,6-吡啶二甲酸为原料,经酰化、酯化、胺解、亲和加成合成2,6-吡啶二甲酰肼-2-羟基萘甲酰腙(L1)。用紫外-可见吸收光谱、荧光光谱、荧光寿命方法研究了酸碱对L1互变异构的影响及L1、Cu(Ⅱ)、铜运输蛋白(copper trafficking protein,apoCopC)三者的相互结合。结果表明,在pH=7.4、50 mmol·L^-1 Tris-HCl缓冲条件下,L1可分别与Cu(Ⅱ)、apoCopC结合形成1:1的配合物,条件结合常数分别为3.32×10⁶ mol^-1·L和4.01×10⁵ mol^-1·L;而Cu(Ⅱ)-L1与apoCopC结合形成1:1复合物的条件结合常数为8.09×10⁵ mol^-1·L。荧光共振能量转移、分子对接模拟表明,L1结合在apoCopC的N端,光谱滴定证实L1、Cu(Ⅱ)、apoCopC可形成以Cu(Ⅱ)为中心的三元配合物。

关键词:

2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙;Cu(Ⅱ);铜运输蛋白;配合物;光谱

English

Spectral Studies on Complex of 2,6-Pyridine Diformylhydrazine 2-Hydroxylnaphthene Carboxylic Hydrazone-Cu(Ⅱ)-CopC

1.
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006, China

Corresponding author: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

Received Date: 25 May 2015
Fund Project:
国家自然科学基金(No.20771068,20901048)

高等学校博士学科点专项科研基金(No.20131401110011)资助项目。

Abstract: 2, 6-Pyridine diformylhydrazine 2-hydroxylnaphthene carboxylic hydrazone (L1) was synthesized by acylation, esterification and aminolysis reaction. The effect of pH value on isomerization of L1 and the binding among L1, Cu(Ⅱ) and copper trafficking protein, apoCopC, were studied by using UV-Vis absorption, fluorescence spectra and fluorescence lifetime measurement. The results show that the binding constant is 3.32×10⁶ mol^-1·L for Cu(Ⅱ)-L1, 4.01×10⁵ mol^-1·L for L1-apoCopC, separately, in pH value of 7.4, 50 mmol·L^-1 Tris-HCl. Meanwhile, the binding constant between Cu(Ⅱ)-L1 and apoCopC is 8.09×10⁵ mol^-1·L. The average distance between the bound L1 and Trp83 of apoCopC from fluorescence resonance energy transfer was determined and the binding site of L1 in apoCopC, which locats at N-terminal, was shown by an automated public domain software package ArgusLab 4.0.1. The formation of a ternary complex, L1-Cu(Ⅱ)-CopC, is confirmed by the titration of Cu(Ⅱ) to L1-apoCopC in pH value of 7.4 and 50 mmol·L^-1 Tris-HCl.

Key words:

2,6-pyridine diformylhydrazine 2-hydroxylnaphthene carboxylic hydrazone;copper(Ⅱ);copper trafficking protein;complex;spectra

1. [1] Arnesano F, Banci L, Bertini I, et al. Structure, 2002,10:1337-1347[1] Arnesano F, Banci L, Bertini I, et al. Structure, 2002,10:1337-1347
2. [2] Arnesano F, Banci L, Bertini I, et al. Proc. Natl. Acad. Sci. USA, 2003,100:3814-3819[2] Arnesano F, Banci L, Bertini I, et al. Proc. Natl. Acad. Sci. USA, 2003,100:3814-3819
3. [3] Zhang L, Koay M, Maher M J, et al. J. Am. Chem. Soc., 2006,128:5834-5850[3] Zhang L, Koay M, Maher M J, et al. J. Am. Chem. Soc., 2006,128:5834-5850
4. [4] Song Z, Ming J, Yang B. J. Biol. Inorg. Chem., 2014,19:359-374[4] Song Z, Ming J, Yang B. J. Biol. Inorg. Chem., 2014,19:359-374
5. [5] Song Z, Zheng X, Yang B. Protein Sci., 2013,22:1519-1530[5] Song Z, Zheng X, Yang B. Protein Sci., 2013,22:1519-1530
6. [6] Song Z, Wang J, Yang B. Spectrachim. Acta A, 2014,118:454-460[6] Song Z, Wang J, Yang B. Spectrachim. Acta A, 2014,118:454-460
7. [7] Zhao Y, Zhang B. New J. Chem., 2006,30:1207-1213[7] Zhao Y, Zhang B. New J. Chem., 2006,30:1207-1213
8. [8] Wang J L, Zhao Y Q, Yang B S. Inorg. Chim. Acta, 2013, 409:484-496[8] Wang J L, Zhao Y Q, Yang B S. Inorg. Chim. Acta, 2013, 409:484-496
9. [9] WANG Cai-Rong(王彩荣), WANG Jing-Lin(王璟琳), LIU Bing(刘斌), et al. Chinese J. Inorg. Chem.(无机化学学报), 2013,29:283-288[9] WANG Cai-Rong(王彩荣), WANG Jing-Lin(王璟琳), LIU Bing(刘斌), et al. Chinese J. Inorg. Chem.(无机化学学报), 2013,29:283-288
10. [10] AN Ming(安鸣), XU Cai-Hong(许彩虹), MENG Jing-Yan(孟晶岩), et al. J. Shanxi. Univ.:Nat. Sci. Ed.(山西大学学报:自然科学版), 2015,8:120-124[10] AN Ming(安鸣), XU Cai-Hong(许彩虹), MENG Jing-Yan(孟晶岩), et al. J. Shanxi. Univ.:Nat. Sci. Ed.(山西大学学报:自然科学版), 2015,8:120-124
11. [11] Wang Z J, Ren L X, Zhao Y Q, et al. Spectrochim. Acta A, 2008,70:892-897[11] Wang Z J, Ren L X, Zhao Y Q, et al. Spectrochim. Acta A, 2008,70:892-897
12. [12] Pang E G, Zhao Y Q, Yang B S. Chin. Sci. Bull., 2005, 50:2302-2305[12] Pang E G, Zhao Y Q, Yang B S. Chin. Sci. Bull., 2005, 50:2302-2305
13. [13] Zheng X Y, Pang E G, Li H Q, et al. Chin. Sci. Bull., 2007,52:743-747[13] Zheng X Y, Pang E G, Li H Q, et al. Chin. Sci. Bull., 2007,52:743-747

扫一扫看文章

计量

PDF下载量: 1
文章访问数: 364
HTML全文浏览量: 25

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙-Cu(Ⅱ)-CopC三元配合物的光谱研究

通讯作者: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

English

Spectral Studies on Complex of 2,6-Pyridine Diformylhydrazine 2-Hydroxylnaphthene Carboxylic Hydrazone-Cu(Ⅱ)-CopC

Corresponding author: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

2,6-吡啶二甲酰肼2-羟基萘甲醛酰腙-Cu(Ⅱ)-CopC三元配合物的光谱研究

通讯作者: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

English

Spectral Studies on Complex of 2,6-Pyridine Diformylhydrazine 2-Hydroxylnaphthene Carboxylic Hydrazone-Cu(Ⅱ)-CopC

Corresponding author: 杨斌盛,E-mail:yangbs@sxu.edu.cn,Tel:0351-7016358

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs