引用本文:
Dong Ju, Sheng Mei Song, Yan Bo Wu, Shao Min Shuang, Chuan Dong. Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin[J]. Chinese Chemical Letters,
2010, 21(4): 480-483.
doi:
10.1016/j.cclet.2009.11.042
Citation: Dong Ju, Sheng Mei Song, Yan Bo Wu, Shao Min Shuang, Chuan Dong. Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin[J]. Chinese Chemical Letters, 2010, 21(4): 480-483. doi: 10.1016/j.cclet.2009.11.042
Citation: Dong Ju, Sheng Mei Song, Yan Bo Wu, Shao Min Shuang, Chuan Dong. Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin[J]. Chinese Chemical Letters, 2010, 21(4): 480-483. doi: 10.1016/j.cclet.2009.11.042
Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin
摘要:
The interaction mechanism between human serum albumin(HSA)and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2-pyrazoline(PFEP)was investigated by fluorescence and absorption titration techniques in combination with molecular modeling method.Stern-Volmer plots at different temperatures proved that PFEP could quench the intrinsic fluorescence of HSA attributed to a static quenching procedure.The association constants were calculated in the range of 1×105-8×105 mol-1 at different pH conditions,and the stoicbiometric ratio of binding was 1:1 between PEEP and HSA.Molecular modeling study showed that the distance between indole moiety of the Trp214 residue and the carbazole group at the terminal of PFEP was 4.45 Å in the optimal model.
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关键词:
- Fluorescent probe
- / Human serum albumin
- / ICT
English
Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin
Abstract:
The interaction mechanism between human serum albumin(HSA)and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2-pyrazoline(PFEP)was investigated by fluorescence and absorption titration techniques in combination with molecular modeling method.Stern-Volmer plots at different temperatures proved that PFEP could quench the intrinsic fluorescence of HSA attributed to a static quenching procedure.The association constants were calculated in the range of 1×105-8×105 mol-1 at different pH conditions,and the stoicbiometric ratio of binding was 1:1 between PEEP and HSA.Molecular modeling study showed that the distance between indole moiety of the Trp214 residue and the carbazole group at the terminal of PFEP was 4.45 Å in the optimal model.
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Key words:
- Fluorescent probe
- / Human serum albumin
- / ICT
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