引用本文:
G. Rezaei Behbehani, A. A. Saboury, M. Mohebbian, S. Ghammamy. Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease[J]. Chinese Chemical Letters,
2010, 21(4): 457-460.
doi:
10.1016/j.cclet.2009.10.021
Citation: G. Rezaei Behbehani, A. A. Saboury, M. Mohebbian, S. Ghammamy. Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease[J]. Chinese Chemical Letters, 2010, 21(4): 457-460. doi: 10.1016/j.cclet.2009.10.021
Citation: G. Rezaei Behbehani, A. A. Saboury, M. Mohebbian, S. Ghammamy. Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease[J]. Chinese Chemical Letters, 2010, 21(4): 457-460. doi: 10.1016/j.cclet.2009.10.021
Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease
摘要:
Cyanide ion was studied as an effector of Jack bean urease(JBU)at 300 K in 30 mmol/L Tris buffer,pH 7 by isothermal titration calorimetry(ITC).The simple novel model was used for CN-+JBU interaction over the whole range of CN-concentrations.The binding parameters recovered from the simple novel model were attributed to the cyanide ion interaction.It was found that cyanide ion acted as a noncooperative inhibitor of JBU,and there is a set of 12 identical and independent binding sites for CN-ions.The dissociation equilibrium constant is 750 μmol/L.The molar enthalpy of binding is △H=-13.6 kJ mol-1.The technique used provided an accurate and quick assessment of the effectiveness of the compounds to inhibit Jack bean urease.
English
Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease
Abstract:
Cyanide ion was studied as an effector of Jack bean urease(JBU)at 300 K in 30 mmol/L Tris buffer,pH 7 by isothermal titration calorimetry(ITC).The simple novel model was used for CN-+JBU interaction over the whole range of CN-concentrations.The binding parameters recovered from the simple novel model were attributed to the cyanide ion interaction.It was found that cyanide ion acted as a noncooperative inhibitor of JBU,and there is a set of 12 identical and independent binding sites for CN-ions.The dissociation equilibrium constant is 750 μmol/L.The molar enthalpy of binding is △H=-13.6 kJ mol-1.The technique used provided an accurate and quick assessment of the effectiveness of the compounds to inhibit Jack bean urease.
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Key words:
- Isothermal titration calorimetry
- / Cyanide ion
- / Jack bean ttrease
- / Binding parameter
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