引用本文:
Wei Zhang, Tao Yang, Da Ming Huang, Kui Jiao. Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film[J]. Chinese Chemical Letters,
2008, 19(5): 589-591.
doi:
10.1016/j.cclet.2008.03.012
Citation: Wei Zhang, Tao Yang, Da Ming Huang, Kui Jiao. Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film[J]. Chinese Chemical Letters, 2008, 19(5): 589-591. doi: 10.1016/j.cclet.2008.03.012
Citation: Wei Zhang, Tao Yang, Da Ming Huang, Kui Jiao. Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film[J]. Chinese Chemical Letters, 2008, 19(5): 589-591. doi: 10.1016/j.cclet.2008.03.012
Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film
摘要:
A novel electrochemical DNA biosensor based on zinc oxide (ZnO) nanoparticles and multi-walled carbon nanotubes (MWNTs)for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite filmmodified glassy carbon electrode (MWNTs/ZnO/CHIT/GCE) was fabricated and DNA probes were immobilized on the electrodesurface. The hybridization events were monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as anindicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the.transgenic corn, with adetection limit of 2.8×10-12 mol/L of target sequence.
English
Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film
Abstract:
A novel electrochemical DNA biosensor based on zinc oxide (ZnO) nanoparticles and multi-walled carbon nanotubes (MWNTs)for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite filmmodified glassy carbon electrode (MWNTs/ZnO/CHIT/GCE) was fabricated and DNA probes were immobilized on the electrodesurface. The hybridization events were monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as anindicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the.transgenic corn, with adetection limit of 2.8×10-12 mol/L of target sequence.
-
Key words:
- ZnO nanoparticles
- / Multi-walled carbon nanotubes
- / Chitosan
- / DNA sensor
- / PAT gene
扫一扫看文章
计量
- PDF下载量: 0
- 文章访问数: 779
- HTML全文浏览量: 0
下载:
