引用本文:
De Bin Zhu, Da Xing, Ya Bing Tang. Highly sensitive ECL-PCR method for detection of K-ras point mutation[J]. Chinese Chemical Letters,
2007, 18(2): 198-200.
doi:
10.1016/j.cclet.2006.12.017
Citation: De Bin Zhu, Da Xing, Ya Bing Tang. Highly sensitive ECL-PCR method for detection of K-ras point mutation[J]. Chinese Chemical Letters, 2007, 18(2): 198-200. doi: 10.1016/j.cclet.2006.12.017
Citation: De Bin Zhu, Da Xing, Ya Bing Tang. Highly sensitive ECL-PCR method for detection of K-ras point mutation[J]. Chinese Chemical Letters, 2007, 18(2): 198-200. doi: 10.1016/j.cclet.2006.12.017
Highly sensitive ECL-PCR method for detection of K-ras point mutation
摘要:
A highly sensitive electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for K-ras point mutation detection is developed. Briefly, K-ras oncogene was amplified by a Ru(bpy)32+ (TBR)-labeled forward and a biotin-labeled reverse primer,and followed by digestion with MvaI restriction enzyme, which only cut the wild-type amplicon containing its cutting site. The digested product was then adsorbed to the streptavidin-coated microbead through the biotin label and detected by ECL assay. The experiment results showed that the different genotypes can be clearly discriminated by ECL-PCR method. It is useful in point mutation detection, due to its sensitivity, safety, and simplicity.
English
Highly sensitive ECL-PCR method for detection of K-ras point mutation
Abstract:
A highly sensitive electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for K-ras point mutation detection is developed. Briefly, K-ras oncogene was amplified by a Ru(bpy)32+ (TBR)-labeled forward and a biotin-labeled reverse primer,and followed by digestion with MvaI restriction enzyme, which only cut the wild-type amplicon containing its cutting site. The digested product was then adsorbed to the streptavidin-coated microbead through the biotin label and detected by ECL assay. The experiment results showed that the different genotypes can be clearly discriminated by ECL-PCR method. It is useful in point mutation detection, due to its sensitivity, safety, and simplicity.
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