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紫外分光光度法表征Lipozyme TL IM脂肪酶转酯化活性

付显锋 ,  郑建永 ,  应向贤 ,  鄢洪德 ,  汪钊

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引用本文: 付显锋, 郑建永, 应向贤, 鄢洪德, 汪钊. 紫外分光光度法表征Lipozyme TL IM脂肪酶转酯化活性[J]. 催化学报, 2014, 35(4): 553-559. doi: 10.1016/S1872-2067(14)60053-X shu
Citation:  Xianfeng Fu, Jianyong Zheng, Xiangxian Ying, Hongde Yan, Zhao Wang. Investigation of Lipozyme TL IM-catalyzed transesterification using ultraviolet spectrophotometric assay[J]. Chinese Journal of Catalysis, 2014, 35(4): 553-559. doi: 10.1016/S1872-2067(14)60053-X shu

紫外分光光度法表征Lipozyme TL IM脂肪酶转酯化活性

    通讯作者: 汪钊
  • 基金项目:

    国家高技术研究发展计划(863计划, 2011AA02A210). (863计划, 2011AA02A210)

摘要: 建立了一种新的有机相脂肪酶转酯化活性测定方法. 以正己烷为溶剂, 脂肪酶催化棕榈酸对硝基苯酯和正丁醇的转酯化反应为模型反应, 通过测定反应液中310 nm下吸光值的变化计算反应转化率. 以气相色谱法对新建的紫外分光光度法进行验证, 分别采用这两种方法测定了七种商品化脂肪酶的转酯化活性, 两种方法所得实验结果基本一致. 利用紫外分光光度检测法考察了Lipozyme TL IM脂肪酶催化转酯化的时间进程及合成活性与酶量的关系, 并对Lipozyme TL IM催化转酯化的性质(最适溶剂、酰基受体特异性、醇耐受性、最优反应温度和热力学稳定性)进行了表征.

English

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  • 发布日期:  2014-04-20
  • 收稿日期:  2013-11-15
  • 网络出版日期:  2014-01-07
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