引用本文:
Li SHEN, Bo Hui XIONG, Run Zhi CONG, Jun De WANG. Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography[J]. Chinese Chemical Letters,
1999, 10(7): 583-586.
Citation: Li SHEN, Bo Hui XIONG, Run Zhi CONG, Jun De WANG. Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography[J]. Chinese Chemical Letters, 1999, 10(7): 583-586.
Citation: Li SHEN, Bo Hui XIONG, Run Zhi CONG, Jun De WANG. Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography[J]. Chinese Chemical Letters, 1999, 10(7): 583-586.
Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography
摘要:
Rigid PSDVB microbeads have been modified with poly（vinyl alcohol) (PVA) adsorbed on their surface to produce an affinny medium. Then Cibacron Blue F3GA was covalentlv attached to the supports. The initial concentration of PVA has effect on the adsorption of PVA. The non-specific interaction of bovine serum albumin (BSA) on the microbeads decreases、、ith the PVA adsorbed. The pH stability test shows that the aftinitv medium is stable up to pH 11.0. And it has specific interaction with lysoryme, but not with pepsin.
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关键词:
- PSDVB microbeads
- / PVA
- / affinity medium
- / Cibacron Blue F3GA
English
Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography
Abstract:
Rigid PSDVB microbeads have been modified with poly（vinyl alcohol) (PVA) adsorbed on their surface to produce an affinny medium. Then Cibacron Blue F3GA was covalentlv attached to the supports. The initial concentration of PVA has effect on the adsorption of PVA. The non-specific interaction of bovine serum albumin (BSA) on the microbeads decreases、、ith the PVA adsorbed. The pH stability test shows that the aftinitv medium is stable up to pH 11.0. And it has specific interaction with lysoryme, but not with pepsin.
-
Key words:
- PSDVB microbeads
- / PVA
- / affinity medium
- / Cibacron Blue F3GA
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