引用本文:
Xiang Hong LI, Yuan MA, Yi CHEN, Yu Fen ZHAO. RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer[J]. Chinese Chemical Letters,
1997, 8(1): 53-56.
Citation: Xiang Hong LI, Yuan MA, Yi CHEN, Yu Fen ZHAO. RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer[J]. Chinese Chemical Letters, 1997, 8(1): 53-56.
Citation: Xiang Hong LI, Yuan MA, Yi CHEN, Yu Fen ZHAO. RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer[J]. Chinese Chemical Letters, 1997, 8(1): 53-56.
RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer
摘要:
In the presence of histidine.N-(O,O-diisopropyl) phosphoryl serine could catalyze the cleavage of RNA in aqueous solution at neutral pH.The results were detected by sub-marine agarose gel electrophoresis and capillary zone electrophoresis.It was found that at high cpncentration histidine could cleave the RNA slightly.While the participation of DIPP-Ser could significantly accelerate the cleavage reaction.N-phosphodipeptide-N-(O.O-diisopropyl) phosphoryl Ser-His are proposed to be involved in the mechanism.
English
RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer
Abstract:
In the presence of histidine.N-(O,O-diisopropyl) phosphoryl serine could catalyze the cleavage of RNA in aqueous solution at neutral pH.The results were detected by sub-marine agarose gel electrophoresis and capillary zone electrophoresis.It was found that at high cpncentration histidine could cleave the RNA slightly.While the participation of DIPP-Ser could significantly accelerate the cleavage reaction.N-phosphodipeptide-N-(O.O-diisopropyl) phosphoryl Ser-His are proposed to be involved in the mechanism.
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