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Citation: LI Jing, ZHU Cheng-Cheng, HE Wei-Jiang. A Turn-on pH Fluorescent Probe Based on NBD-NH2 Fluorophore and Its pHi Imaging Application[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(12): 2528-2534. doi: 10.3969/j.issn.1001-4861.2013.00.396 shu

A Turn-on pH Fluorescent Probe Based on NBD-NH2 Fluorophore and Its pHi Imaging Application

  • 1.

    State Key Laboratory of Coordination Chemistry, School of Chemistry andChemical Engineering, Nanjing University, Nanjing 210093, China

  • Received Date: 20 May 2013
    Available Online: 16 July 2013

    Fund Project: 国家自然科学基金(No.21271100) (No.21271100)国家重点基础研究发展计划(No.2011CB935800)资助项目。 (No.2011CB935800)

  • A fluorescent probe of PET mechanism, NBD-Py, was constructed by coupling fluorophore NBD-NH2 with a proton receptor N-(4-pyridylmethyl) ethylene diamine. This probe displays the pH decrease-induced emission enhancement in pH range from 5.0 to 8.5, with a pKa of 6.48. This sensing behavior is reversible and not interfered by normal intracellular metal cations such as Na+, K+, Ca2+, Mg2+ and other transition metal cations. Comparison between the pH sensing behaviors of NBD-Py and its analogue without the N-(4-pyridyl)methyl group disclosed that the pyridylmethyl group is essential its pH sensing ability for weak acidic and near neutral pH, which is important for cell biology. Confocal imaging in HeLa cells confirmed the pHi imaging ability and the lysosome-targetability of NBD-Py, which is valuable for lysosome cell biology.
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